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Divergence of chloroplast gene organization in three legumes: Pisum sativum, Vicia faba and Phaseolus vulgaris

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Summary

Isolated chloroplasts from Pisum sativum were found to contain at least 32 tRNA species. Hybridization of in vitro labeled, identified, chloroplast tRNAs to Pisum chloroplast DNA fragments revealed the locations of the tRNA genes on the circular chloroplast genome. Comparison of this gene map to the maps of Vicia faba and Phaseolus vulgaris showed that the chloroplast genomes of Pisum and Phaseolus are otherwise more closely related than either genome is to the chloroplast genome of Vicia. Furthermore, the results suggest how possible recombination events could be involved in the evolution of these three closely related, but divergent, chloroplast genomes.

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Author notes

  1. Gerhard Bookjans

    Present address: Department of Agronomy, College of Agriculture, University of Kentucky, 40546-0091, Lexington, KY, U.S.A.

Authors and Affiliations

  1. Department of Genetics, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Copenhagen V, Denmark

    Edwin J. Crouse, Bjarn M. Stummann, Gerhard Bookjans & Knud W. Henningsen

  2. Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, 15 rue Descartes, F-67084, Strasbourg Cedex, France

    Edwin J. Crouse, Mfika Mubumbila & Jacques-Henry Weil

  3. University Department of Biochemistry, BioSciences West, University of Arizona, Tucson, AZ, 85721, U.S.A.

    Christine Michalowski & Hans J. Bohnert

Authors
  1. Edwin J. Crouse
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  2. Mfika Mubumbila
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  3. Bjarn M. Stummann
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  4. Gerhard Bookjans
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  5. Christine Michalowski
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  6. Hans J. Bohnert
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  7. Jacques-Henry Weil
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  8. Knud W. Henningsen
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Crouse, E.J., Mubumbila, M., Stummann, B.M. et al. Divergence of chloroplast gene organization in three legumes: Pisum sativum, Vicia faba and Phaseolus vulgaris . Plant Mol Biol 7, 143–149 (1986). https://doi.org/10.1007/BF00040140

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  • DOI: https://doi.org/10.1007/BF00040140

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