Abstract
Cytoplasmic male-sterile lines CMS89 and CMSBaso of sunflower (Helianthus annuus) differ from the fertile lines HA89 and Baso in a mitochondrial DNA sequence in the vicinity of theatpA gene. In addition, the transcriptional pattern of theatpA gene is changed in male-sterile lines compared to fertile ones. Besides one main transcript in the fertile lines, the male-sterile lines additionally show larger transcripts. Investigation of Baso and CMSBaso revealed that the two fertility-restored lines of CMS89 have the same transcripts as CMSBaso or a combination of CMSBaso and CMS89. Comparing the mitochondrialin organello translation products we observed a unique 16 kDa protein, which is expressed in male-sterile lines carrying theH. petiolaris cytoplasm but is not detectable in fertile lines withH. annuus cytoplasm. The 16 kDa protein can also be observed in restored lines but not inH. petiolaris. As the expression of the 16 kDa polypeptide seems to be linked to the interspecific cross betweenH. petiolaris andH. annuus it may play a role in CMS. By different criteria such as molecular mass, isoelectric point and peptide fingerprinting the α subunit of the F1-ATPase of male-sterile and fertile lines is very similar if not identical.
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Horn, R., Köhler, R.H. & Zetsche, K. A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Mol Biol 17, 29–36 (1991). https://doi.org/10.1007/BF00036803
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DOI: https://doi.org/10.1007/BF00036803