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In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.

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Abstract

Micropropagation of the actinorhizal plant Hippophae rhamnoides L. (sea-buckthorn) was achieved on Murashige and Skoog (MS) medium supplemented with 1 μM of benzylaminopurine (BA). A multiplication frequency of three to five shoots per explant was observed after 28 days. Rooting of these shoots was achieved in a medium containing 1/4 strength MS without growth regulators. The rooted plants were transferred to Turface R artificial substrate and inoculated with pure cultures of two Frankia strains. These plantlets subsequently developed nodules which fixed nitrogen.

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Authors and Affiliations

  1. CRBF, Faculté de Foresterie, Université Laval, G1K 7P4, Ste-Foy, Québec, Canada

    D. Montpetit & M. Lalonde

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  1. D. Montpetit
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  2. M. Lalonde
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Montpetit, D., Lalonde, M. In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.. Plant Cell Tiss Organ Cult 15, 189–199 (1988). https://doi.org/10.1007/BF00033643

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  • DOI: https://doi.org/10.1007/BF00033643

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