Abstract
It has been suggested previously that non-photochemical quenching of chlorophyll fluorescence is associated with a decrease in the rate of photosystem 2 (PS 2) photochemistry. In this study analyses of fluorescence yield changes, induced by flashes in leaves exhibiting different amounts of non-photochemical quenching of fluorescence, are made to determine the effect of non-photochemical excitation energy quenching processes on the rate of PS 2 photochemistry. It is demonstrated that both the high-energy state and the more slowly relaxing components of non-photochemical quenching reduce the rate of PS 2 photochemistry. Flash dosage response curves for fluorescence yield show that non-photochemical quenching processes effectively decrease the relative effective absorption cross-section for PS 2 photochemistry. It is suggested that non-photochemical quenching processes exert an effect on the rate of PS 2 photochemistry by increasing the dissipation of excitation energy by non-radiative processes in the pigment matrices of PS 2, which consequently results in a decrease in the efficiency of delivery of excitation energy for PS 2 photochemistry.
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Abbreviations
- F:
-
fluorescence yield (subscripts o, i, m, s, v, f define minimal, i, maximal, steady-state, variable and single turnover flash induced levels, respectively)
- ΔF:
-
1 s saturating pulse-induced fluorescence yield change
- ΔFf :
-
single turnover flash-induced fluorescence yield change
- ΔFfmax :
-
single turnover saturating flash-induced fluorescence yield change
- PPFD:
-
photosynthetic photon flux density
- PQ:
-
plastoquinone
- PS 2:
-
photosystem 2
- QA :
-
primary quinone-type electron acceptor of PS 2
- qE :
-
energy-dependent quenching of chlorophyll fluorescence
- qN :
-
non-photochemical quenching of chlorophyll fluorescence
- qP :
-
photochemical quenching of chlorophyll fluorescence
References
Bouges Bocquet B (1973) Electron transfer between the two photosystems in spinach chloroplasts. Biochim Biophys Acta 314: 250–256
Bradbury M and Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem 2 electron acceptors and fluorescence emission from photosystems 1 and 2. Biochim Biohys Acta 63: 542–551
Chylla RA, Casals GV and Whitmarsh J (1987) Evidence for slow turnover in a fraction of photosystem 2 complexes in thylakoid membranes. Biochim Biophys Acta 894: 562–71
Crofts AR and Wraight C (1983) The electrical domain of photosynthesis. Biochim Biophys Acta 726: 149–185
Delosme R (1967) Etude de l'induction de fluorescence des algues vertes et des chloroplastes au début d'une illumination intense. Biochim Biophys Acta 143: 108–126
Duysens LNM (1979) Transfer and trapping of excitation energy in photosystem 2. In: Chlorophyll Organization and Energy Transfer in Photosynthesis. CIBA Foundation Symposium 61 (new series): pp 323–340. Elsevier Biomedical Press, Amsterdam
Falkowski PG, Wyman K, Ley AC and Mauzerall DC (1986) Relationship of steady-state photosynthesis to fluorescence in eukaryotic algae. Biochim Biophys Acta 849: 183–192
Genty B, Briantais JM and Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochem Biophys Acta 990: 87–92
Horton P and Hague A (1988) Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts. IV. Resolution of non-photochemical quenching. Biochim Biophys Acta 932: 107–115
Kitijima M and Butler WL (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta 376: 105–115
Krause GH, Vernotte C and Briantais JM (1982) Photo-induced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Biochim Biophys Acta 679: 116–124
Mauzerall DC (1972) Light-induced changes in Chlorella, and the primary photoreactions for the production of oxygen. Proc Natl Acad Sci USA 69: 1358–1362
Mauzerall DC (1978) Multiple excitations and the yield of chlorophyll a fluorescence in photosynthetic system. Photochem Photobiol 28: 991–998
Morin P (1984) Etude des cinétiques de fluorescence de la chlorophylle in vivo, dans les premiers instants qui suivent le début de l'illumination. J Chim Phys 61: 674–680
Neubauer C and Schreiber U (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: I. Saturation characteristics and partial control by the photosystem 2 acceptor side. Z Naturforsch 42c: 1246–1254
Robinson HH and Crofts AR (1983) Kinetics of the oxidation reduction reactions of the photosystem 2 quinone acceptor complex and the pathway for deactivation. FEBS Lett 155: 221–226
Schatz GH, Brock H and Holzwarth AR (1987) Picosecond kinetics of fluorescence and absorbance changes in photosystem 2 particles excited at low photon density. Proc Natl Acad Sci USA 84: 8414–8418
Schreiber U (1986) Defection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorimeter. Photosynth Res 9: 261–272
Schreiber U and Neubauer C (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: II. Partial control of the photosystem 2 donor side and possible ways of interpretation. Z Naturforsch 42C: 1255–1264
Schreiber U, Schliwa U and Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62
Velthuys BR and Amesz J (1974) Charge accumulation at the reducing side of system 2 of photosynthesis. Biochim Biophys Acta 333: 85–94
Vernotte C, Etienne AL and Briantais J-M (1979) Quenching of the system 2 chlorophyll fluorescence by the plastoquinone pool. Biochim Biophys Acta 406: 465–478
Weis E and Berry JA (1987) Quantum efficiency of photosystem 2 in relation to energy dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 189–208
Zankel R (1973) Rapid fluorescence changes observed in chloroplasts: their relationship to the O2 evolving system. Biochim Biophys Acta 325: 138–148
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Genty, B., Harbinson, J., Briantais, JM. et al. The relationship between non-photochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves. Photosynth Res 25, 249–257 (1990). https://doi.org/10.1007/BF00033166
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DOI: https://doi.org/10.1007/BF00033166