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Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA

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Abstract

We introduced a plant selection marker, nptII, to the left of border A in the Agrobacterium Ti plasmid pTiA6. Infection of tobacco leaf discs with the modified Agrobacterium strain gave rise to kanamycin-resistant calli which grew in a hormone-dependent manner. Southern hybridization analysis of DNA isolated from four transformants indicated initiation of DNA transfer at or near border A and absence of T-DNA sequences. These results demonstrate that DNA transfer events starting at a left border on a native Ti plasmid and moving away from the T-DNA region occur and that they can be detected by designing a suitable selection strategy.

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  1. Department of Plant Molecular Biology, School of Biotechnology, Madurai Kamaraj University, 625 021, Madurai, India

    Vai. Ramanathan & K. Veluthambi

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  1. Vai. Ramanathan
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  2. K. Veluthambi
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Ramanathan, V., Veluthambi, K. Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA. Plant Mol Biol 28, 1149–1154 (1995). https://doi.org/10.1007/BF00032676

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  • DOI: https://doi.org/10.1007/BF00032676

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