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Analysis of inhibition of photosynthesis due to water stress in the C3 species Hordeum vulgare and Vicia faba: Electron transport, CO2 fixation and carboxylation capacity

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Abstract

A C3 monocot, Hordeum vulgare and C3 dicot, Vicia faba, were studied to evaluate the mechanism of inhibition of photosynthesis due to water stress. The net rate of CO2 fixation (A) and transpiration (E) were measured by gas exchange, while the true rate of O2 evolution (J O2) was calculated from chlorophyll fluorescence analysis through the stress cycle (10 to 11 days). With the development of water stress, the decrease in A was more pronounced than the decrease in J O2 resulting in an increased ratio of Photosystem II activity per CO2 fixed which is indicative of an increase in photorespiration due to a decrease in supply of CO2 to Rubisco. Analyses of changes in the J O2 A ratios versus that of CO2 limited photosynthesis in well watered plants, and RuBP pool/RuBP binding sites on Rubisco and RuBP activity, indicate a decreased supply of CO2 to Rubisco under both mild and severe stress is primarily responsible for the decrease in CO2 fixation. In the early stages of stress, the decrease in C i (intercellular CO2) due to stomatal closure can account for the decrease in photosynthesis. Under more severe stress, CO2 supply to Rubisco, calculated from analysis of electron flow and CO2 exchange, continued to decrease. However, C i, calculated from analysis of transpiration and CO2 exchange, either remained constant or increased which may be due to either a decrease in mesophyll conductance or an overestimation of C i by this method due to patchiness in conductance of CO2 to the intercellular space. When plants were rewatered after photosynthesis had dropped to 10–30% of the original rate, both species showed near full recovery within two to four days.

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Abbreviations

A-:

net CO2 assimilation rate

A *-:

net CO2 assimilation rate plus dark respiration

ATP-:

adenosine triphosphate

CABP-:

carboxyarabinitol 1,5-bisphosphate

C a-:

ambient CO2 concentration

C c-:

CO2 concentration in the chloroplast

C i-:

intercellular CO2 concentration

E-:

transpiration rate

g m-:

mesophyll conductance

g s-:

stomatal conductance

J O2 :

true rate of O2 evolution

LSD-:

least significant difference

PPFD-:

photosynthetic photon flux density

PS II-:

Photosystem II

R n-:

dark respiration rate

Rubisco-:

ribulose 1,5-bisphosphate carboxylase/oxygenase

RuBP-:

ribulose 1,5-bisphosphate

RWC-:

relative water content

νc-:

rate of carboxylation

νo-:

rate of oxygenation

ϕPSII-:

quantum yield of Photosystem II

Γ-:

CO2 compensation point in the absence of R n

Ψ-:

water potential

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Authors and Affiliations

  1. Department of Botany, Washington State University, 99164-4238, Pullman, WA, USA

    Archana Lal, M. S. B. Ku & Gerald E. Edwards

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  1. Archana Lal
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  2. M. S. B. Ku
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  3. Gerald E. Edwards
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Lal, A., Ku, M.S.B. & Edwards, G.E. Analysis of inhibition of photosynthesis due to water stress in the C3 species Hordeum vulgare and Vicia faba: Electron transport, CO2 fixation and carboxylation capacity. Photosynth Res 49, 57–69 (1996). https://doi.org/10.1007/BF00029428

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  • DOI: https://doi.org/10.1007/BF00029428

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