Abstract
We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the amino and carboxyl termini located on the stromal membrane surface, is predicted. This structure is similar to that predicted for CP 47, a PS II chlorophyll a binding protein (Bricker T (1990) Photosynth Res 24: 1–13). The model is discussed in reference to the possible location of chlorophyll binding sites.
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This work was supported by National Institutes of Health Research Grant, GM40703 and U.S. Department of Energy Grant, DE-FG01-92ER20076 (to R.T.S.).
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Sayre, R.T., Wrobel-Boerner, E.A. Molecular topology of the Photosystem II chlorophyll a binding protein, CP 43: Topology of a thylakoid membrane protein. Photosynth Res 40, 11–19 (1994). https://doi.org/10.1007/BF00019041
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DOI: https://doi.org/10.1007/BF00019041