Abstract
Cloned DNA of the geminivirus wheat dwarf virus (WDV) was successfully used to infect seedling wheat plants. The clone was derived from circular double-stranded viral DNA isolated from naturally infected tissue. The initiation of infection was mediated by Agrobacterium tumefaciens using cloned dimeric WDV genomes in a binary Agrobacterium vector. The WDV DNA which comprised the infectious clone was sequenced and is compared with the published sequence of a Swedish isolate of the same virus. The results confirm that the single WDV genome component of 2.75 kb carries all the information necessary for production of viral symptoms, virus particles and viral double- and single-stranded DNA forms.
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References
Bevan M: Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12: 8711–8721 (1984).
Bock KR, Guthrie EJ, Woods RD: Purification of maize streak virus and its relationship to viruses associated with streak diseases of sugar cane and Panicum maximum. Ann Appl Biol 77: 289–296 (1974).
Boulton MI, Markham PG: In: Jones HC, Torrance L (eds) Developments and Applications in Virus Testing. Association of Applied Biologists, Wellesbourne, UK (1986).
Brown DM, Frampton J, Goelet P, Karn J: Sensitive detection of RNA using strand-specific M13 probes. Gene 20: 139–144 (1982).
Ditta G, Stanfield S, Corbin D, Helinki DR: Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347–7351 (1980).
Dlabola J: Warning as regards a new disease of wheat. Za Vysokou Urodu 17: 403–405 (1960).
Dollet M, De Taffin G: Progress report on the virological study of the New Hebrides coconut disease, 1. Dec. 1978. IRHO Document 1437(a) (1979).
Dollet M, Accotto GP, Lisa V, Menissier J, Boccardo G: A geminivirus, serologically related to maize streak virus, from Digitaria sanguinalis from Vanuatu. J Gen Virol 67: 933–937 (1986).
Donson J, Accotto GP, Boulton MI, Millineaux PM, Davies JW: The nucleotide sequence of a geminivirus from Digitaria sanguinalis. Virology 161: 160–169 (1987).
Donson J, Gunn HV, Woolston CJ, Pinner MS, Boulton MI, Mullineaux PM, Davies JM: Agrobacterium-mediated infectivity of cloned digitaria streak virus DNA. Virology 162: 248–250 (1988).
Feinberg AP, Vogelstein B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).
Francki RIB, Hatta T: Chloris striate mosaic virus. In: Descriptions of Plant Viruses, No 221 Commonwealth Mycological Institute/Association of Applied Biologists (1980).
Girvitz SC, Bacchetti S, Rainbow AJ, Graham FL: A rapid and efficient procedure for the purification of DNA from agarose gels. Anal Biochem 106: 492–496 (1980).
Graves ACF, Goldman SL: The transformation of Zea mays seedlings with Agrobacterium tumefaciens. Detection of T-DNA specific enzyme activities. Plant Mol Biol 7: 43–50 (1986).
Grimsley N, Hohn B, Hohn T, Walden R: “Agroinfection”, an alternative route for viral infection of plants by using the Ti-plasmid. Proc Natl Acad Sci USA 83: 3282–3286 (1986).
Grimsley N, Hohn T, Davies JW, Hohn B: Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325: 177–179 (1987).
Hepburn AG, White J, Pearson L, Maunders MJ, Clarke LE, Prescott AG, Blundy KS: The use of pNJ5000 as an intermediate vector for the genetic manipulation of Agrobacterium Ti-plasmids. J Gen Microbiol 131: 2961–2969 (1985).
Hille J, Dekker M, Luttikhuis HO, Van Kammen A, Zabel P: Detection of T-DNA transfer to plant cells by A. tumefaciens virulence mutants using agroinfection. Mol Gen Genet 205: 411–416 (1986).
Hooykaas-Van Slogteren GMS, Hooykaas PJJ, Schilperoort RA: Expression of Ti-plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens. Nature 311: 763–764 (1984).
Howell SH: Physical structure and genetic organisation of the genome of maize streak virus (Kenyan isolate). Nucleic Acids Res 12: 7359–7375 (1984).
Howell SH: Corrigendum: Physical structure and genetic organisation of maize streak virus (Kenyan isolate). Nucleic Acids Res 13: 3018–3019 (1985).
Lindsten K, Lindsten B, Abdelmoeti M, Juntti N: Purification and some properties of wheat dwarf virus. In: Proceedings of the 3rd Conference on Virus Diseases of Gramineae in Europe. Rothamsted Experimental Station, Harpenden, UK pp 27–31 (1980).
Macdowell SW, Macdonald H, Hamilton WDO, Coutts RHA, Buck KW: The hucleotide sequence of cloned wheat dwarf virus DNA. EMBO J 4: 2173–2180 (1985).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Marsh JL, Erfle M, Wykes EJ: The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481–485 (1984).
Maule AJ, Hull R, Donson J: The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. J Virol Meth 6: 215–224 (1983).
Maxam AM, Gilbert W: Sequencing of end-labeled DNA with base-specific chemical cleavages. In: Grossman L, Moldave K (eds) Methods in Enzymology Vol. 65, pp 499–560. Academic Press, New York (1980).
Mullineaux PM, Donson J, Morris-Krsinich BAM, Boulton MI, Davies JW: The nucleotide sequence of maize streak virus DNA. EMBO J 3: 3063–3068 (1984).
Mumford DL: A new method of mechanically transmitting curly top virus. Phytopathology 62: 1217–1218 (1972).
Mumford DL: Purification of curly top virus. Phytopathology 64: 136–139 (1974).
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA: Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing J Mol Biol 143: 161–178 (1980).
Severin HHP: Curly leaf transmission experiments. Phytopathology 14: 80–93 (1924).
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517 (1975).
Stanley J: The molecular biology of geminiviruses. Adv Virus Res 30: 139–177 (1985).
Stanley J, Markham PG, Callis RJ, Pinner MS: The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO J 5: 1761–1767 (1986).
Townsend R, Watts J, Stanley J: Synthesis of viral DNA forms in Nicotiana plumbaginifolia protoplasts inoculated with cassava latent virus (CLV); evidence for the independent replication of one component of the CLV genome. Nucleic Acids Res 14: 1253–1265 (1986).
Vacke J: Host plants, range and symptoms of wheat dwarf virus. Vedecke Prace Vyzk Ustavu Rostl Vroby, Praha-Ruzyne 17: 151–162 (1972).
Yanisch-Perron C, Vieira J, Messing J: Improved M13 cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene 33: 103–119 (1985).
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Woolston, C.J., Barker, R., Gunn, H. et al. Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA. Plant Mol Biol 11, 35–43 (1988). https://doi.org/10.1007/BF00016012
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DOI: https://doi.org/10.1007/BF00016012