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The CRISPR/Cas9 System as a Tool to Engineer Chromosomal Translocation In Vivo

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Chromosome Translocation

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1044))

Abstract

The CRISPR/Cas9 system has emerged as a powerful tool to edit the genome. Among many applications, the system generates the exciting possibility of engineering small and large portions of chromosomes to induce a variety of structural alterations such as deletions, inversions, insertions and inter-chromosomal translocations. Furthermore, the availability of viral vectors that express Cas9 has been critical to deliver the CRISPR/Cas9 system directly in vivo to induce chromosomal rearrangements. This review provides an overview of the state-of-the-art CRISPR/Cas9 technology to model a variety of rearrangements in vivo in animal models.

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Acknowledgments

This work was supported by National Institute of Health grants R01 CA196703-01 and R01 CA222598-01 to R.C.; Associazione Italiana per la Ricerca sul Cancro (AIRC) grant IG-12023 to R.C.; Worldwide Cancer Research (former AICR) grant 12-0216 to R.C.; National Research Foundation of Korea (NRF) fellowship 2016R1A6A3A03006840 to T.C.C.

Author Information

The authors declare no competing financial interests. Correspondence and requests for materials should be addressed to RC. (roberto.chiarle@childrens.harvard.edu).

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Author notes

  1. Authors Taek-Chin Cheong and Rafael B. Blasco have equally contributed to this chapter.

Authors and Affiliations

  1. Department of Pathology, Boston Children’s Hospital, Boston, MA, USA

    Taek-Chin Cheong, Rafael B. Blasco & Roberto Chiarle

  2. Harvard Medical School, Boston, MA, USA

    Taek-Chin Cheong, Rafael B. Blasco & Roberto Chiarle

Authors
  1. Taek-Chin Cheong
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  2. Rafael B. Blasco
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  3. Roberto Chiarle
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Corresponding author

Correspondence to Roberto Chiarle .

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Editors and Affiliations

  1. National Institute of Biological Sciences, Beijing, China

    Yu Zhang

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Cheong, TC., Blasco, R.B., Chiarle, R. (2018). The CRISPR/Cas9 System as a Tool to Engineer Chromosomal Translocation In Vivo. In: Zhang, Y. (eds) Chromosome Translocation. Advances in Experimental Medicine and Biology, vol 1044. Springer, Singapore. https://doi.org/10.1007/978-981-13-0593-1_4

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