Skip to main content
SpringerLink
Log in

Conditional lethal markers: spontaneous haploid selection in plants

  • Chapter
In Vitro Haploid Production in Higher Plants

Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 23))

Abstract

Haploidy refers to any organism, tissue or cell, having the chromosomal constitution similar to the normal gametes of a given species (Chase, 1951). Haploid plants are useful for developing inbred lines (Gallais, 1986; Fouroughi-Wehr and Wenzel, 1990) and for the selection of recessive mutations at the cellular level (Grafe et al., 1986; Marion-Poll et al., 1988). Haploids plants can be produced by two methods: a) in vitro, and b) in situ.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aberg, B., 1947. On the mechanism of the toxic action of chlorate and some related substance upon young wheat plants. Ann. R. Agric. Coll. Sweden 15: 37–107.

    Google Scholar 

  • Akiyoshi, D.E., H. Klee, R.M. Amasino, E.W. Nester and M.P. Gordon, 1984. T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proc. Natl. Acad. Sci. USA 81: 5994–5998.

    Google Scholar 

  • Al Yasiri, S. and O.M. Rogers, 1971. Attempting chemical induction of haploidy using toluidine blue. J. Am. Soc. Hort. Sci. 96: 126–127.

    Google Scholar 

  • Bajaj, Y.P.S., 1990. In vitro production of haploids and their use in cell genetics and plant breeding. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 12, Haploids in Crop Improvement 1, pp 3–44. Springer-Verlag, Berlin/Heidelberg/New York.

    Google Scholar 

  • Banga, S.S. and K.S. Labana, 1983. Incidence of parthenogenetic maternais after prickle pollination of Brassica juncea (L.) Coss. Z. Pflanzenzüchtg. 91: 227–232.

    Google Scholar 

  • Banga, S.S., 1986. Hybrid pollen-aided induction of matromorphy in Brassica. Z. Pflanzenzüchtg. 96: 86–89.

    Google Scholar 

  • Bennett, M.D., R.A. Finch and I.R. Barclay, 1976. The time rate and mechanism of chromosome elimination in Hordeum hybrids. Chromosoma 54: 175–200.

    Article  Google Scholar 

  • Blakeslee, A., J. Belling, M.E. Farnham and A.D. Berger, 1922. A haploid mutant Datura stramonium. Science 55: 646–647.

    Article  PubMed  CAS  Google Scholar 

  • Budar, F., F. Deboeck, M. Van Montagu and J.P. Hernalsteens, 1988. Introduction and expression of the octopine T-DNA oncogenes in tobacco plants and their progeny. Plant Sci. 46: 195–206.

    Article  Google Scholar 

  • Burk, L.G., 1962. Haploids in genetically marked progenies of tobacco. J. Hered. 53: 222–225. Burk, L.G., D.U. Gerstel and E.A. Wernsman, 1979. Maternal haploid of Nicotiana tabacum L. from seed. Science 206: 585.

    Google Scholar 

  • Camilleri, C. and L. Jouanin, 1991. The TR-DNA region carrying the auxin synthesis genes of Agrobacterium rhizogenes agropine-type plasmid pRiA4: nucleotide sequence analysis and introduction into tobacco plants. Mol. Plant-Microbe Interact. 4: 155–162.

    Google Scholar 

  • Chase, S.S., 1951. The monoploid method of developing inbred lines. In: Proc. 6th Annual Hybrid Corn. Industry–Research Conf., Chicago, 1951, Nov. 28–29, pp. 29–34.

    Google Scholar 

  • Chase, S.S., 1952. Production of homozygous diploids of maize from monoploids. Agron. J. 44: 263–267.

    Google Scholar 

  • Chase, S.S., 1963. Androgenesis–its use for transfer of maize cytoplasm. J. Hered. 54: 152–158.

    Article  Google Scholar 

  • Chase, S.S., 1969. Monoploids and monoploid-derivatives of maize (Zea mays L.). Bot. Rev. 35: 117–167.

    Google Scholar 

  • Chen B.Y. and W.K. Heenen, 1989. Evidence for spontaneous diploid androgenesis in Brassica napus L. Sex. Plant Reprod. 2: 15–17.

    Google Scholar 

  • Chilton, M.D., H.G.J. Drummond, D.J. Merlo, D. Sciaky, A.L. Montoya, M.P. Gordon and E.W. Nester, 1977. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11: 263–271.

    Article  PubMed  CAS  Google Scholar 

  • Clausen, R.E. and M.C. Mann, 1924. Inheritance in Nicotiana tabacum. V. The occurrence of haploid plants in interspecific progenies. Proc. Natl. Acad. Sci. USA 10: 121–124.

    Google Scholar 

  • Clausen, R.E. and W.E. Lammerts, 1929. Interspecific hybridization in Nicotiana. Haploid and diploid merogony. Am. Nat. 43: 279–282.

    Google Scholar 

  • Coe, E.H., 1959. A line of maize with high haploid frequency. Am. Nat. 93: 381–382. Coe, E.H. and S. Sarkar, 1964. The detection of haploids in maize. J. Hered. 55: 231–233.

    Google Scholar 

  • Collins, G.B., P.D. Legg and M.J. Kasperbauer, 1974. Use of anther-derived haploids in Nicotiana. I. Isolation of breeding lines differing in total alkaloid content. Crop Sci. 14: 77–80.

    Google Scholar 

  • De Buyser, J., Y. Henry, R. Laur and P. Lonnet, 1981. Utilisation de l’androgenèse in vitro dans les programmes de sélection du blé tendre (Triticum aestivum L.). Z. Pflanzenzüchtg. 87: 290–299.

    Google Scholar 

  • Devaux, P., 1992. Haploidy in barley and wheat improvement. In: Reproductive Biology and Plant Breeding. XIIIth Eucarpia Congress, July 6–11th, 1992, Angers, pp. 140–151.

    Google Scholar 

  • Doré, C., 1989. Obtention de plantes haploïdes de chou cabus (Brassica oleracea L. ssp. capitata) après culture in vitro d’ovules pollinisés par du pollen irradié. C.R. Acad. Sci. Paris, Série D 309: 729–734.

    Google Scholar 

  • Doré, C., 1976. Doublement du stock chromosomique d’haploïdes d’asperge par culture in vitro des méristèmes en présence de colchicine. Ann. Amélior. Plantes 26: 647–653.

    Google Scholar 

  • Doré, C., 1974. Production de plantes homozygotes mâles et femelles à partir d’anthères d’asperge cultivées in vitro. C.R. Acad. Sci. Paris 278: 2135–2138.

    Google Scholar 

  • Dumas de Vaulx, R., 1979. Obtention de plantes haploïdes chez le melon (Cucumis melo L.) après pollinisation par Cucumis ficifolius A. Rich. C.R. Acad. Sci. Paris, Série D 289: 875–878.

    Google Scholar 

  • Ecochard, R., M.S. Ramanna and D. De Nettancourt, 1969. Detection analysis of tomato haploids. Genetica 40: 181–190.

    Article  Google Scholar 

  • Ehrensberger, R., 1948. Versuche zur Auslösung von Haploidie bei Blütenpflanzen. Biol. Zentralbl. 67: 537–543.

    Google Scholar 

  • Fouroughi-Wehr, B. and G. Wenzel, 1990. Recurrent selection alternating with haploids steps - a rapid breeding procedure for combining agronomic traits in inbreeders. Theor. Appl. Genet. 80: 564–568.

    Google Scholar 

  • Fouroughi-Wehr, B. and W. Friedt, 1984. Rapid production of recombinant barley yellow mosaic virus-resistant Hordeum vulgare lines by anther culture. Theor. Appl. Genet. 67: 377–382.

    Google Scholar 

  • Gaines, E.F. and H.C. Aase, 1926. A haploid wheat plant. Am. J. Bot. 13: 373–385.

    Google Scholar 

  • Gallais, A, 1986. Place de l’haploïdisation dans les schémas de sélection. Le Sélectionneur Français 36: 47–58.

    Google Scholar 

  • Gerassimova, H., 1936. Experimentell erhaltene haploide pflanze von Crepis tectorum L. Planta 25: 696–702.

    Article  Google Scholar 

  • Goodsell, S.F.,1961. Male sterility in corn by androgenesis. Crop Sci. 1: 227–228.

    Google Scholar 

  • Grafe, R., A. Marion-Poll and M. Caboche, 1986. Improved in vitro selection of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia. Theor. Appl. Genet. 73: 299–304.

    Google Scholar 

  • Gresshoff, P.M. and C.H. Doy, 1972. Development and differenciation of haploid Lycopersicon esculentum (tomato). Planta 107: 161–170.

    Article  Google Scholar 

  • Hamza, S., C. Camilleri, J.M. Pollien, H. Vaucheret, J.P. Bourgin and Y. Chupeau, 1993. Selection for spontaneous tomato haploids using a conditional lethal marker. Theor. Appl. Genet. 86: 657–664.

    Google Scholar 

  • Harland, S.C., 1936. Haploids in polyembryonic seeds of Sea Island cotton. J. Hered. 27: 229–231.

    Google Scholar 

  • Hille, J., M. Koornneef, M.S. Ramanna and P. Zabel, 1989. Tomato: a crop amenable to improvment by cellular and molecular methods. Euphytica 42: 1–23.

    Article  CAS  Google Scholar 

  • Hoffmann, F., E. Thomas and G. Wenzel, 1982. Anther culture as a breeding tool in rape. II. Progeny analysis of androgenetic lines and induced mutants from haploid cultures. Theor. Appl. Genet. 61: 225–232.

    Google Scholar 

  • Horlow, C., M.C. Defrance, J.M. Pollien, J. Goujaud, R. Delon and G. Pelletier, 1993. Transfer of cytoplasmic male sterility by spontaneous androgenesis in tobacco (Nicotiana tabacum L.). Euphytica 66: 45–53.

    Article  Google Scholar 

  • Hougas, R. W., S.J. Peloquin and A.C. Gabert, 1964. Effect of seed parent and pollinator on frequency of haploids in Solanum tuberosum. Crop Sci. 4: 593–595.

    Article  Google Scholar 

  • Hu, H., 1985. Use of haploids in crop improvement. In: Proc. Intl. Seminar Biotechnology in International Agriculture, pp. 75–84. IRRI, Los Banos.

    Google Scholar 

  • Illies, Z.M., 1964. The occurrence of haploid seedlings in P. abies. Naturwissenschaften 51: 442.

    Article  Google Scholar 

  • Illies, Z.M., 1974. Induction of haploid parthenogenesis in aspen by post-pollination treatment with toluidine blue. Sylvae Geneticae 23: 221–226.

    Google Scholar 

  • Inzé, D., A. Follin, M. Van Lijsebettens, C. Simoens, C. Genetello and M. Van Montagu, 1984. Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole-3-acetic acid synthesis. Mol. Gen. Genet. 194: 265–274.

    Google Scholar 

  • Johanson, D.A., 1934. Haploids in Hordeum vulgare. Proc. Natl. Acad. Sci. USA 20: 98–100. Jörgensen, C.A., 1928. The experimental formation of heteroploid plants in the genus Solanum. J. Genet. 19: 133–211.

    Google Scholar 

  • Kappert, H., 1933. Erbliche Polyembryonie bei Linum usitatissimum. Biol. Zentralblatt. 53: 276–307.

    Google Scholar 

  • Kappert, H., 1950. Botanische Untersuchiengen zur Erblichkeit der Polyembryonie. Moderne Biologie. F.W. Peters, Berlin.

    Google Scholar 

  • Kasha, K.J. and K.N. Kao, 1970. High frequency haploid production in barley (Hordeum vulgare L.). Nature (London) 225: 874–876.

    Article  CAS  Google Scholar 

  • Keller, W.A., K.C. Armstrong and A.I. de la Roche, 1983. The production and utilization of microspore-derived haploids in Brassica crops. In: S.K. Sen and K.L. Giles (Eds.), Plant Cell Culture in Plant Improvement, pp. 169–183. Plenum, New York.

    Google Scholar 

  • Kermicle, J.L., 1969. Androgenesis conditioned by a mutation in maize. Science 166: 1422–1424.

    Article  PubMed  CAS  Google Scholar 

  • Kimber, G. and R. Riley, 1963. Haploids angiosperms. Bot. Rev. 29: 480–531.

    Google Scholar 

  • Klee, H.J., R.B. Horsch, M.A. Hinchee, M.B. Hein and N.L. Hoffmann, 1987. The effects of overproduction of two Agrobacterium tumefaciens T-DNA auxin biosynthesis gene products in transgenic petunia plants. Gene and Development 1: 86–96.

    Article  CAS  Google Scholar 

  • Koornneef, M., J.A.M. van Diepen, C.J. Hanhart, A.C. Kiebem-de Waart, L. Martinelli, H.C.H. Schoenmakers and J. Wijbrandi, 1989. Chromosomal instability in cell and tissue cultures of tomato haploids and diploids. Euphytica 43: 179–186.

    Article  Google Scholar 

  • Kostoff, D., 1929. An androgenetic Nicotiana haploid. Z. Zellforsch. 9: 640–642.

    Article  Google Scholar 

  • Kuo, C.S., W.L. Lu and Y.L. Kui, 1986. Corn (Zea mays L.): Production of pure lines through anther culture. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 2, Crops 1, pp. 152–164. Springer-Verlag, Berlin/Heidelberg/New York/Tokyo.

    Google Scholar 

  • Lacadena, J.R., 1974. Spontaneous and induced parthenogenesis and androgenesis. In: K.J. Kasha (Ed.), Haploids in Higher Plants: Advances and Potential. pp. 13–32, University of Guelph, Guelph.

    Google Scholar 

  • Laurie, D.A. and M.D. Bennett, 1988. The production of haploid wheat plants from wheat x - maize crosses. Theor. Appl. Genet. 76: 393–397.

    Google Scholar 

  • Leblanc, H. and J.V. Escalant, 1992. Induced parthenogenesis in Musa spp. In: XIII Eucarpia congress, Angers, pp. 181–182.

    Google Scholar 

  • Levieil, C. and C. Huyghe, 1985. Observation des gamètogenèses mâle et femelle, de la fécondation et de la formation d’embryons non-zygotiques après éclaircissage des anthères et sacs embryonnaires de Cichorium intybus L. et de Linum usitissimum L. C.R. Acad. Sci. Paris 20: 769–774.

    Google Scholar 

  • Marion-Poll, A., C. Missonier, J. Goujaud and M. Caboche, 1988. Isolation and characterization of valine-resistant mutants of Nicotiana plumbaginifolia. Theor. Appl. Genet. 75: 272–277.

    Article  Google Scholar 

  • Morgan, D.T., J.R. Rappleye and R.D. Rappleye, 1950. Twin and triplet pepper seedlings. A study of polyembryony in Capsicum frutescens. J. Hered. 41: 91–95.

    PubMed  Google Scholar 

  • Morinaga, T. and E. Fukushima, 1931. Preliminary report on the haploid plant of rice, Oryza sativa L. Proc. Imper. Acad. 7: 383–384.

    Google Scholar 

  • Muller, J.F., J. Goujaud and M. Caboche, 1985. Isolation in vitro of naphthalenacetic acid-tolerant mutants of Nicotiana tabacum, which are impaired in root morphogenesis. Mol. Gen. Genet. 199: 194–200.

    Google Scholar 

  • Nakamura, A., T. Yamada, N. Katodani, R. Itagaki and M. Oka, 1984. Studies on the haploid method of breeding in tobacco. SABRAO J. 6: 107–131.

    Google Scholar 

  • Nussaume, L., M. Vincentz and M. Caboche, 1991. Constitutive nitrate reductase: a dominant conditional marker for plant genetics. Plant J. 1: 267–274.

    Article  Google Scholar 

  • Pelletier, G., M. Férault, J. Goujaud, F. Vedel and M. Caboche, 1987. The use of rootless mutants for the screening of spontaneous androgenetic and gynogenetic haploids in Nicotiana tabacum: evidence for the direct transfert of cytoplasm. Theor. Appl. Genet. 75: 13–15.

    Google Scholar 

  • Randolph, L.F., 1932. Some effects of high temperature on polyploidy and other variations in maize. Proc. Natl. Acad. Sci. USA 18: 222–229.

    Google Scholar 

  • Raquin, C., 1985. Induction of haploid plants by in vitro culture of Petunia ovaries pollinated with irradiated pollen. Z. Pflanzenzüchtg. 94: 166–169.

    Google Scholar 

  • Raquin, C., A. Cornu, E. Farcy, D. Maizonnier, G. Pelletier and F. Vedel, 1989. Nucleus substitution between Petunia species using gamma ray-induced androgenesis. Theor. Appl. Genet. 78: 337–341.

    Google Scholar 

  • Rode, J.C. and R. Dumas de Vaulx, 1987. Obtention de plantes haploïdes de carottes (Daucus carota L.) issues de parthénogenèse induite par du pollen irradié et culture in vitro des graines immatures. C.R. Acad. Sci. Paris 305: 225–229.

    Google Scholar 

  • Sarkar, K.R., J.K.S. Sachan and D.S. Mathur, 1972. Detection of androgenetic monoploids in maize. Curr. Sci. 41: 190–192.

    Google Scholar 

  • Sauton, A. and R. Dumas de Vaulx, 1987. Obtention de plantes haploïdes chez le melon (Cucumis melo L.) par gynogenèse induite par du pollen irradié. Agronomie 7: 141–148.

    Article  Google Scholar 

  • Schaeffer, G.W. and P.S. Baenziger, 1982. Anther culture and pollen plant regeneration in wheat (Triticum aestivum L. em Thell). In: A. Fujiwara (Ed.), Plant Tissue Culture 1982, pp. 553–556. Maruzen, Tokyo.

    Google Scholar 

  • Schell, J., M. Van Montagu, M. De Beuckeler, M. De Block, A. Depicker, M. De Wilde, G. Engler, C. Genetello, J.P. Hernalsteens, M. Holster, J. seurinck, B. Silva, F. Van Vliet and R. Villarroel, 1979. Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host. Proc. R. Soc. London B. 204: 251–266.

    Google Scholar 

  • Schröder, G., S. Waffenschmidt, E.W. Weiler and J. Schröder, 1984. The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur. J. Biochem. 138: 387391.

    Google Scholar 

  • Sharp, W.R., R.S. Raskin and H.E. Sommer, 1972. The use of nurse cultures in the development of haploid clones in tomato. Planta 104: 357–361.

    Article  Google Scholar 

  • Snape, J.W. and E. Simpson, 1986. The utilization of double haploid lines in quantitative genetics. Bull. Soc. Bot. Fr. 133, Actualites Bot. 4: 59–66.

    Google Scholar 

  • Snape, J.W., 1989. Doubled haploid breeding: theorical basis and applications. In: Proc. 2nd Int. Symp. Genetic Manipulation in Crops, pp. 19–30. CIMMYT, Mexico.

    Google Scholar 

  • Subrahmanyam, N.C. and K.J. Kasha, 1973. Selective chromosomal elimination during haploid formation in barley follwing interspecific hybridization. Chromosoma 42: 111–125.

    Article  Google Scholar 

  • Symko, S., 1969. Haploid barley from crosses of Hordeum bulbosum (2 x) x Hordeum vulgare (2 x). Can. J. Genet. Cytol. 11: 602–608.

    Google Scholar 

  • Thévenin, L., 1968. Les problèmes d’amélioration chez Asparagus officinalis. II. Haploïdie et amélioration. Ann. Amélior. Plantes, 18: 327–365.

    Google Scholar 

  • Van Onckelen, H., P. Rüdelsheim, D. Inzé, A. Follin, E. Messens, S. Horemans, J. Schell and M. Van Montagu, 1985. Tobacco plants transformed with the Agrobacterium T-DNA gene 1 contain high amounts of indole-3-acetamide. FEBS Lett. 181: 373–376.

    Article  Google Scholar 

  • Vaucheret, H., P. Mourrain, J.C. Robalo and J.M. Pollien. Gene silencing as a tool to select for spontaneous haploid plants (submitted).

    Google Scholar 

  • Vincentz, M. and M. Caboche, 1991. Constitutive expression of nitrate reductase allows growth and development of Nicotiana plubaginifolia plants. EMBO J. 10: 1027–1035.

    CAS  Google Scholar 

  • Wenzel, G., C. Meyer, T. Przewozny, H. Uhrig and O. Schieder, 1980. Incorporation of microspore and protoplast techniques into potato breeding programs. In: E.D. Earle and Y. Demarly (Eds.), NSF-CNRS SEM on Regeneration from Cells and Tissue Culture and Genetic Variability, Orsay, pp. 290–302.

    Google Scholar 

  • Wenzel, G and H. Uhrig, 1981. Breeding for nematode and virus resistance in potato via anther culture. Theor. Appl. Genet. 59: 333–340.

    Google Scholar 

  • Wu, J.L., L.Q. Zhong, F.H. Nong, M.L. Chen, H.Y. Zhang and B.L. Zheng, 1983. Selection of a pure line of maize (Zea mays) by anther culture and observations on its hybrids. Sci. Sinica 26: 725–733.

    Google Scholar 

  • Zagorska, N., M. Abdjieva, C. Georgiev and R. Georgieva, 1982. Morphogenesis and plant differentiation in anther cultures of the genus Lycopersicon Mill. In: A. Fujiwara (Ed.), Plant Tissue Culture, pp. 539–540. Maruzen, Tokyo.

    Google Scholar 

  • Zamir, D., R.A. Jones and N. Kedar, 1980. Anther culture of male-sterile tomato (Lycopersicon esculentum Mill) mutants. Plant Sci. Lett. 21: 223–227.

    Google Scholar 

  • Zamir, D., S.D. Tanksley and R.A. Jones, 1981. Genetic analysis of the origin of plants regenerated from anther tissues of Lycopersicon esculentum Mill. Plant Sci. 21: 223–227.

    Google Scholar 

  • Zhang, Y.X., Y. Lespinasse and E. Chevreau, 1988. Obtention de plantes haploïdes de pommier (Malus x domestica Borkh.) issues de parthénogenèse induite in situ par du pollen irradié et culture in vitro des pépins immatures. C.R. Acad. Sci. Paris, Série D 305: 451–457.

    Google Scholar 

Download references

Authors
  1. C. Horlow
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Hamza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Chupeau
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Pelletier
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Plant Production Department, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    S. K. Sopory

  3. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    R. E. Veilleux

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Horlow, C., Hamza, S., Chupeau, Y., Pelletier, G. (1996). Conditional lethal markers: spontaneous haploid selection in plants. In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1860-8_17

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-1860-8_17

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4579-9

  • Online ISBN: 978-94-017-1860-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation