The Methodology of Plant Genetic Manipulation: Criteria for Decision Making

1. Front Matter

   Pages i-ix

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2. Special Lecture

   1. Genetic manipulation in plant breeding — prospects and limitations

      • C. N. Law

      Pages 1-12

3. Comparison of genetic transformation techniques

   1. Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment

      • Paul Christou

      Pages 13-27

   2. Transforming the plastome: genetic markers and DNA delivery systems

      • P. J. Dix, T. A. Kavanagh

      Pages 29-34

   3. Genetic engineering of cereal crop plants: a review

      • A. Jähne, D. Becker, H. Lörz

      Pages 35-44

   4. Shoot apical meristems as a target for gene transfer by microballistics

      • C. Sautter, N. Leduc, R. Bilang, V. A. Iglesias, A. Gisel, X. Wen et al.

      Pages 45-51

   5. Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate

      • Indridi Benediktsson, Claudia P. Spampinato, Otto Schieder

      Pages 53-61

   6. Expression of foreign genes in sunflower (Helianthus annuus L.) — evaluation of three gene transfer methods

      • Hélène Laparra, Monique Burrus, Reiner Hunold, Brigitte Damm, Ana-Maria Bravo-Angel, Roberte Bronner et al.

      Pages 63-74

   7. Maize transformation utilizing silicon carbide whiskers: a review

      • J. A. Thompson, P. R. Drayton, B. R. Frame, Kan Wang, J. M. Dunwell

      Pages 75-80

   8. Transgenic barley by particle bombardment. Inheritance of the transferred gene and characteristics of transgenic barley plants

      • Anneli Ritala, Reino Aikasalo, Kristian Aspegren, Marjatta Salmenkallio-Marttila, Satu Åkerman, Leena Mannonen et al.

      Pages 81-88

   9. Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions

      • László Sági, Serge Remy, Bert Verelst, Bart Panis, Bruno P. A. Cammue, Guido Volckaert et al.

      Pages 89-95

   10. Transformation of lily by Agrobacterium

       • Simon A. Langeveld, Merel M. Gerrits, Anton F. L. M. Derks, Piet M. Boonekamp, John F. Bol

       Pages 97-100

   11. An assessment of morphogenic and transformation efficiency in a range of varieties of potato (Solanum tuberosum L.)

       • Philip J. Dale, Kaija K. Hampson

       Pages 101-108

   12. Transgenic apples display stable gene expression in the fruit and Mendelian segregation of the transgenes in the R1 progeny

       • D. J. James, A. J. Passey, S. A. Baker

       Pages 109-112

   13. Transformation studies in Hordeum vulgare using a highly regenerable microspore system

       • W. A. Harwood, S. J. Bean, D.-F. Chen, P. M. Mullineaux, J. W. Snape

       Pages 113-118

   14. Biolistic introduction of a synthetic Bt gene into elite maize

       • M. Hill, K. Launis, C. Bowman, K. McPherson, J. Dawson, J. Watkins et al.

       Pages 119-123

   15. Genetic transformation of Eustoma grandiflorum Griseb. by microprojectile bombardment

       • Laura Semeria, Anna Maria Vaira, Gian Paolo Accotto, Andrea Allavena

       Pages 125-130

   16. Some methodological aspects of apple transformation by Agrobacterium

       • J. G. Schaart, K. J. Puite, L. Kolova, N. Pogrebnyak

       Pages 131-134

4. Target traits for genetic manipulation

   1. Physiological complexity and plant genetic manipulation

      • Marcello Buiatti, Patrizia Bogani

      Pages 135-147

   2. Strategies for engineering virus resistance in transgenic plants

      • T. A. Kavanagh, C. Spillane

      Pages 149-158

A range of novel techniques is available to the plant breeder today to complement classical breeding methods. The new options are based on the integration of advances in plant cell biology with those in plant molecular biology.
Plant cell, tissue and organ cultures provide efficient systems for transformation, for the achievement of wide crosses and for the production of variation through spontaneous and induced mutation, while permitting effective isolation of desired genotypes by in vitro selection.
This book presents a critical appraisal of the methodologies of plant genetic manipulation for advanced undergraduates, postgraduates, researchers and plant breeders, and provides guidance on the choice of breeding options. The latter depends on the breeding system of the crop, the breeding objective and the tissue culture systems applicable to the target genotype(s).

• Genotyp
• Mutation
• biology
• breeding
• plant
• plants

` ... a valuable supplement to every text book in the field of plant genetic manipulation. ... highly recommended. .. very valuable for university teachers who want to provide some basic literature for their graduate students ... '
Journal of Plant Physiology, 150 (1997)
`The book succeeds in its aim of presenting a clear and comprehensive view of highly dynamic area of applied plant science.'
Scientia Horticulturae, 68 (1997)

• Department of Plant Science, University College, Cork, Ireland

  Alan C. Cassells, Peter W. Jones

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