Abstract
One approach to studying the role of galactolipids in thylakoid membranes has been to incubate membranes with lipolytic enzymes and then to determine the relationship between the changes occuring in their lipid content and composition and simultaneously in their photochemical activities1. However, in most studies2–4, galactolipids have been deacylated by means of unspecific lipases which do not allow an unequivocal assessement of function to each lipid. Moreover, it is often difficult to distinguish between the effects due to the hydrolysis of the parent-lipids themselves and to those resulting from hydrolysis products. in this investigation, special conditions were used allowing lipase from Rhizopus arrhizus to selectively hydrolyze MGDG in the outer monolayer of the thylakoid membrane.
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Abbreviations
- BSA:
-
defatted bovine serum albumin
- DAD:
-
diaminodurene
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DCPIP:
-
2,6-dichlorophenolindophenol
- DQH2 :
-
durohydroquinone
- LRa:
-
lipase from Rhizopus arrhizus
- MGDG:
-
monogalactosyldiacylglycerol
- MOPS:
-
3-(N-morpholino)propanesulfonic acid
- TMPD:
-
N,N,N′, N′-tetramethyl-p-phenylenediamine
References
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Siegenthaler, PA., Giroud, C. (1987). Effects of Monogalactosyldiacylglycerol (MGDG) Depletion on Photosynthetic Activities in OAT Thylakoid Membranes. In: Biggins, J. (eds) Progress in Photosynthesis Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3535-8_51
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DOI: https://doi.org/10.1007/978-94-009-3535-8_51
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