Abstract
Over the past 15 years, site-directed genome modification has proven to be an efficient and robust approach. Meganucleases, sequence-specific endonucleases with long recognition sites, represent one of several tools described in this book that can be used for this purpose. This chapter will review the early stages of the technology (with the first strand break-induced gene targeting with I-SceI), and describe the recent advances in protein engineering that have led to the making of tailored meganucleases. We will then summarize the data and strategies available today regarding their use for site-directed genome modification. In the last section, we will discuss the latest data available on Transcription activator like effectors proteins as they have recently emerged as a promising new tool for genome modifications.
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Epinat, J.C., Silva, G.H., Pâques, F., Smith, J., Duchateau, P. (2013). Engineered Meganucleases for Genome Engineering Purposes. In: Renault, S., Duchateau, P. (eds) Site-directed insertion of transgenes. Topics in Current Genetics, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4531-5_6
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