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Stimuli-Sensitive Release of Lysozyme from Hydrogel Containing Phosphate Groups

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Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems

Summary

Large amount of lysozyme could be loaded in hydrogels containing phosphate groups by ionic binding between negatively-charged phosphate groups and positively-charged lysozyme. Lysozyme release from the hydrogels was influenced by the conditions such as ionic strength and pH. The rate of lysozyme release at pH 7.4 was much faster than that at pH 1.4. The lysozyme release at pH 7.4 is based on the ion exchange mechanism due to the ionization of the phosphate groups. The pH-sensitive dissociation of ionic binding resulted in the lysozyme release in response to a step-wise pH change.

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References

  1. Nakamae K, Miyata T, Hoffman AS. (1992) Swelling behavior of hydrogels containing phosphate groups. Makromol Chem 193: 983–990

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  2. Miyata T, Nakamae K, Hoffman AS, Kanzaki Y. (1994) Stimuli-sensitivities of hydrogels containing phosphate groups. Macromol Chem Phys 195: 111–112

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  3. Nagamatsu S, Nabeshima Y, Hoffman AS. (1992) Ion exchange drug delivery system; loading of lysozyme into poly(acrylic acid) hydrogels. ACS Polym Preprints 33(2): 478–479

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Author information

Authors and Affiliations

  1. Faculty of Engineering, Kobe University, Rokko, Nada, Kobe 657, Japan

    Katsuhiko Nakamae & Takeshi Nizuka

  2. Faculty of Engineering, Kansai University, Suita, Osaka 564, Japan

    Takashi Miyata & Tadashi Uragami

  3. Center for Bioengineering, University of Washington, Seattle, WA, 98195, USA

    Allan S. Hoffman

  4. Unichemical Co., Ltd., Ikoma, Nara 636, Japan

    Yoshio Kanzaki

Authors
  1. Katsuhiko Nakamae
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  2. Takeshi Nizuka
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  3. Takashi Miyata
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  4. Tadashi Uragami
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  5. Allan S. Hoffman
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  6. Yoshio Kanzaki
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Editor information

Editors and Affiliations

  1. Department of Chemistry, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo, 102, Japan

    Naoya Ogata Ph.D. (Professor) (Professor)

  2. Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, 84112, USA

    Sung Wan Kim Ph.D. (Professor and Director) (Professor and Director)

  3. Department of Chemical Technology and Institute of Biomedical Technology, University of Twente, P.O. Box 217, 7500 AD, Enschede, The Netherlands

    Jan Feijen Ph.D. (Professor) (Professor)

  4. Institute of Biomedical Engineering, Tokyo Women’s Medical College, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162, Japan

    Teruo Okano Ph.D. (Professor) (Professor)

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© 1996 Springer-Verlag Tokyo

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Nakamae, K., Nizuka, T., Miyata, T., Uragami, T., Hoffman, A.S., Kanzaki, Y. (1996). Stimuli-Sensitive Release of Lysozyme from Hydrogel Containing Phosphate Groups. In: Ogata, N., Kim, S.W., Feijen, J., Okano, T. (eds) Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65883-2_92

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  • DOI: https://doi.org/10.1007/978-4-431-65883-2_92

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-65885-6

  • Online ISBN: 978-4-431-65883-2

  • eBook Packages: Springer Book Archive

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