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High Pressure Inactivation of Yeast and Spheroplasts at Subzero Temperature

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Abstract

In order to reduce pressure level of food application and also to progress in cell mechanisms comprehension, combination of low temperature and high pressure was tested. The yeast Saccharomyces cerevisiae was treated 10 minutes at different temperatures between 25°C and 20°C. Pressurisation at sub-zero temperatures enhanced significantly the effect of pressure. In term of cell inactivation, a decrease of 10°C is equivalent to an increase of 50 MPa. Similar phenomenon has been identified on pressure induced lysis of yeast spheroplasts which suggested that low temperature should enhance perturbations of membrane functions.

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References

  1. Reyns, K.M., et al., Kinetic analysis and modelling of combined high-pressure-temperature inactivation of the yeast Zygosaccharomyces bailii. Int J Food Microbiol, 2000. 56 (2–3): p. 199–210.

    Article  CAS  Google Scholar 

  2. Sonoike, K., et al., Effects of pressure and temperature on the death rates of Lactobacillus casei and Escherichia coli, in High Pressure and Biotechnology, C. Balny, et al., Editors. 1992, INSERM/John Libbey Eurotext Ltd: Montrouge. p. 297–301. High Pressure Inactivation of Yeast and Spheroplasts at Subzero Temperature 393

    Google Scholar 

  3. Takahashi, K., Sterilization of microorganisms by hydrostatic pressure at low temperature, in High Pressure and Biotechnology, C. Balny, et al., Editors. 1992, INSERM/John Libbey Eurotext Ltd: Montrouge. p. 303–307.

    Google Scholar 

  4. Gervilla, R., V. Ferragut, and B. Guamis, High pressure inactivation of microorganisms inoculated into ovine milk of different fat contents. J Dairy Sci, 2000. 83 (4): p. 674–82.

    Article  CAS  Google Scholar 

  5. Hashizume, C., K. Kimura, and R. Hayashi, Kinetic analysis of yeast inactivation by high pressure treatment at low temperatures. Biosci Biotechnol Biochem, 1995. 59 (8): p. 1455–8.

    Article  CAS  Google Scholar 

  6. Smeller, L., Pressure-temperature phase diagrams of biomolecules. Biochim Biophys Acta, 2002. 1595 (1–2): p. 11–29.

    Article  CAS  Google Scholar 

  7. Perrier-Cornet, J.M., M. Hayert, and P. Gervais, Yeast cell mortality related to a high-pressure shift: occurrence of cell membrane permeabilization. J Appl Microbiol, 1999. 87 (1): p. 1–7.

    Article  CAS  Google Scholar 

  8. Ulmer, H.M., et al., Effects of Pressure-Induced Membrane Phase Transitions on Inactivation of HorA, an ATP-Dependent Multidrug Resistance Transporter, in Lactobacillus plantarum. Appl Environ Microbiol, 2002. 68 (3): p. 1088–95.

    Article  CAS  Google Scholar 

  9. Hawley, S.A., Reversible pressure-temperature denaturation of chymotrypsinogen. Biochemistry, 1971. 10 (13): p. 2436–2442.

    Article  CAS  Google Scholar 

  10. Douzals, J.P., et al., Pressure-temperature phase transition diagram for wheat starch. Journal of agricultural and food chemistry, 2001. 49: p. 873–876.

    Article  CAS  Google Scholar 

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© 2003 Springer-Verlag Berlin Heidelberg

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Perrier-Cornet, JM., Tapin, S., Gervais, P. (2003). High Pressure Inactivation of Yeast and Spheroplasts at Subzero Temperature. In: Winter, R. (eds) Advances in High Pressure Bioscience and Biotechnology II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05613-4_70

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  • DOI: https://doi.org/10.1007/978-3-662-05613-4_70

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05674-1

  • Online ISBN: 978-3-662-05613-4

  • eBook Packages: Springer Book Archive

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