Abstract
The ligand binding processes of hemoglobin and myoglobin are among the most extensively studied kinetic mechanisms in biology. In recent years, the photodeligation mechanisms of hemoglobin and myoglobin have been investigated by a number of groups using picosecond absorption spectroscopy [1–4]. In this paper, we present some of our recent results, using a newly developed technique, picosecond time-resolved resonance Raman spectroscopy [5–7], which produces vibrational spectra of transient rather than stable molecules. Flash photolysis techniques, such as picosecond absorption spectroscopy, have been powerful methods for identifying chemical intermediates and providing kinetic rate information. The broad absorptions though, contain only limited structural information. Resonance Raman spectroscopy, on the other hand, is capable of providing detailed vibrational information [8] and can be time-resolved using pulsed laser techniques [9].
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
C.V. Shank, E.P. Ippen, and R. Bersohn, Science, 193, 50 (1976).
L.J. Noe, W.G. Eisert, and P.M. Rentzepis, Proc. Natl. Acad. Sci. USA, 76, 573 (1978).
B.J. Greene, R.M. Hochstrasser, R.B. Weisman, and W.A. Eaton, Proc. Natl. Acad. Sci. USA, 75, 5255 (1978).
D.A. Chernoff, R.M. Hochstrasser, and A.W. Steele, Proc. Natl. Acad. Sci. USA, 77, 5606 (1980).
J. Terner, T.G. Spiro, M. Nagumo, M.F. Nicol, and M.A. El-Sayed, J. Amer. Chem. Soc., 102, 3238 (1980).
M. Coppey, H. Tourbez, P. Valat, and B. Alpert, Nature (London) 284, 568 (1980).
J. Terner, J. Stong, T.G. Spiro, M. Nagumo, M.F. Nicol, M.A. El-Sayed, Proc. Natl. Acad. Sci. USA, 78, 1313 (1981).
T.G. Spiro, Acc. Chem. Res., 7, 339 (1974).
J. Terner, A. Campion, and M.Â. El-Sayed, Proc. Natl. Acad. Sci. USA, 74, 5121 (1977).
K.B. Lyons, J.M. Friedman, and P.A. Fleury, Nature (London) 275, 565 (1978).
M.F. Perutz, Brit. Med. Bull., 32, 195 (1976).
P. Stein, J. Terner, and T.G. Spiro, J. Phys. Chem., in press (1982).
C.A. Sawicki and Q. H. Gibson, J. Biol. Chem., 251, 1533 (1975).
R.B. Miles, G. Laufer, C. Paddock, and G. Faris, Applied Optics, 19, 3593 (1980).
A. Campion, J. Terner, and M.A. El-Sayed, Nature (London) 265, 659 (1977).
J. Terner, C.-L. Hsieh, and M.A. El-Sayed, Biophys. J., 26, 527 (1979).
J. Terner, C.-L. Hsieh, A.R. Burns, and M.A. El-Sayed, Proc. Natl. Acad. Sci. USA, 76, 3046 (1979).
M. Nagumo, M. Nichol, and M.A. El-Sayed, J. Phys. Chem., 85, 2435 (1981).
M. Abe, T. Kitagawa, and Y. Kyogoku, J. Chem. Phys., 69, 4526 (1978).
S. Choi, T.G. Spiro, K.C. Langry, K.M. Smith, D.L. Budd and G.N. LaMar (1982) submitted for publication.
C.A. Reed, T. Mashiko, W.R. Scheidt, K. Spartalian and G. Land, J. Amer. Chem. Soc., 102, 2302 (1980).
T.G. Spiro, and T.C. Strekas, J. Amer. Chem. Soc., 96, 338 (1974).
T. Kitagawa, T. Iizuka, M. Saito, and Y. Kyogoku, Chem. Lett. (1975) 849.
T.G. Spiro and J.M. Burke, J. Amer. Chem. Soc., 98, 5482 1976 ).
N.M. Ksenoforitova, W.G. Maslov, A.N. Sidorov, and Ya. S. Bobovich, Opt. Spectrosc., 40, 462 (1976).
P.A. Cornelius, A.W. Steele, D.A. Chernoff, and R.M. Hochstrasser, Proc. Natl. Acad. Sci. USA, 78, 7526 (1981).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Terner, J., Spiro, T.G., Voss, D.F., Paddock, C., Miles, R.B. (1982). Picosecond Time-Resolved Resonance Raman Spectroscopy of the Photolysis Product of Oxy-Hemoglobin. In: Eisenthal, K.B., Hochstrasser, R.M., Kaiser, W., Laubereau, A. (eds) Picosecond Phenomena III. Springer Series in Chemical Physics, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87864-0_79
Download citation
DOI: https://doi.org/10.1007/978-3-642-87864-0_79
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-87866-4
Online ISBN: 978-3-642-87864-0
eBook Packages: Springer Book Archive