Abstract
Although as recently as 10 to 15 years ago plant scientists seldom made use of antibodies as research tools, that is no longer the case. Antibodies are quickly becoming indispensible for many purposes, including one-step purification of antigens, their visualization in situ by immunocytochemistry and on nitrocellulose blots of polyacrylamide gels, and their quantitation by radioimmunoassay or enzyme immunoassay. The relatively recent development of monoclonal antibodies produced by hybridoma technology (Köhler and Milstein 1975; see Coding 1983 for thorough treatment) expands the utility of antibodies as research tools by at least an order of magnitude. Whereas a typical antiserum, which is conveniently referred to as a polyclonal antibody preparation, can be specific for a given antigen, a monoclonal antibody is specific for a given domain, known as an antigenic determinant or epitope, on that antigen. Consequently, while polyclonal antibodies provide information about the location or quantity of an antigen, a monoclonal antibody provides information about the location or quantity of a small portion of that antigen. Furthermore, a panel of monoclonal antibodies directed to a single, complex antigen, such as a protein, can be used to dissect that antigen and to identify its structure-function relationships.
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Abbreviations
- ELISA:
-
enzyme-linked immunosorbent assay
- Ig:
-
immunoglobulin
- kD:
-
kilodalton
- PAGE:
-
polyacrylamide gel electrophoresis
- PBS:
-
10 mM sodium phosphate, 140 mM NaC1, pH 7.4
- Pr and Pfr:
-
phytochrome in the red- and far-red-absorbing forms, respectively
- SDS:
-
sodium dodecyl sulfate
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Pratt, L.H., McCurdy, D.W., Shimazaki, Y., Cordonnier, MM. (1986). Immunodetection of Phytochrome: Immunocytochemistry, Immunoblotting, and Immunoquantitation. In: Linskens, HF., Jackson, J.F. (eds) Immunology in Plant Sciences. Modern Methods of Plant Analysis, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82853-9_3
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DOI: https://doi.org/10.1007/978-3-642-82853-9_3
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