Determination of Transferrin (Tf) and Group-Specific Component (Gc) Subtypes on a Single Polyacrylamide Gel with an Immobilized pH Gradient

Abstract

With isoelectric focusing the simultaneous determination of multiple systems — routinely done in starch gel electrophoresis — is usually not possible. The consequent increase in labor and costs is a non-neglectable fact for many laboratories. The ease in generating stable, linear and ultranarrow pH gradients with Immobiline is helpful to develop methods for the simultaneous typing of focusing systems that have isoelectric points that lie within a half of a pH unit. The allelic products of Gc and Tf have iPs between 4.95 – 5.4. A method using immobilized pH gradients to type the complete sets of phenotypes of Tf and Gc in one run on polyacrylamide is presented.