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Encyclopedia of Cancer pp 1718–1721Cite as

Hollow Fiber Assay

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Definition

The hollow fiber assay (HFA) is a fast in vivo assay to determine the cytotoxic effect of drugs, as well as their pharmacodynamic (Pharmacodynamics) effects on human tumor cell lines grown in hollow fibers that are implanted subcutaneously or intraperitoneally in mice or rats.

Characteristics

Various in vivo models exist to assess the efficacy of potential anticancer agents, including subcutaneously implanted xenografts, orthotopic models, and the HFA. The HFA has been optimized at the National Cancer Institute (NCI) and is a unique in vivo model for drug discovery. It permits the simultaneous evaluation of the efficacy of anticancer agents against multiple tumor cell lines in two physiological compartments of one animal. In this assay, semipermeable biocompatible fibers are used, which can be filled with cancer cells. These cancer cells can be derived from different tissue origins and can have different cellular characteristics. The fibers are made of polyvinylidene...

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References

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Author information

Authors and Affiliations

  1. Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands

    Irene V. Bijnsdorp & Godefridus J. Peters

Authors
  1. Irene V. Bijnsdorp
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  2. Godefridus J. Peters
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Corresponding author

Correspondence to Irene V. Bijnsdorp .

Editor information

Editors and Affiliations

  1. University Professor of Genetics, German Cancer Research Center - DKFZ, Heidelberg, Germany

    Manfred Schwab

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© 2011 Springer-Verlag Berlin Heidelberg

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Bijnsdorp, I.V., Peters, G.J. (2011). Hollow Fiber Assay. In: Schwab, M. (eds) Encyclopedia of Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16483-5_2786

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