Abstract
The nonpathogenic Alphaproteobacterium Rhizobium radiobacter (syn. Agrobacterium tumefaciens) (RrF4) is a subculture of the endobacterium R. radiobacter, which is intricately associated with its host, the beneficial plant-colonizing fungal basidiomycete Piriformospora indica. RrF4 is genetically very similar to the well-studied plant pathogenic R. radiobacter biovar I strain C58 (genomovar G8). Highly similar genetic content of RrF4 and C58 denotes the high potential for RrF4 to directly interact with plants. The failure to cure P. indica from its endobacterium still hampers a conclusive prediction of the bacterium’s extended role in the interaction of the fungus with a broad spectrum of host plants. However, beneficial activities shown in cereals and the Brassicaceae Arabidopsis thaliana were hardly distinguishable when induced either by RrF4 or P. indica. We discuss here the various strategies employed to detect, characterize, and eventually elucidate the endobacterium’s role in the tripartite symbiosis with its fungal host and a plant.
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Acknowledgment
This work was supported by the German Science Foundation (DFG) to KHK (KO 1208/24-1) and PK (DFG KA 875/8-1). We acknowledge technical assistance by the Bioinformatics Core Facility at JLU Giessen and access to resources supported by the Bundesministerium fﺰr Bildung und Forschung (BMBF) grant FKZ 031A533 to AG within the de.NBI network.
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Glaeser, S.P. et al. (2017). Detection and Characterization of Endobacteria in the Fungal Endophyte Piriformospora indica . In: Varma, A., Sharma, A. (eds) Modern Tools and Techniques to Understand Microbes. Springer, Cham. https://doi.org/10.1007/978-3-319-49197-4_15
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