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3D Printing and Biofabrication pp 1–34Cite as

Fabrication and Printing of Multi-material Hydrogels

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Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

Abstract

Bioprinting has emerged over the past decade as a prominent technology in the field of tissue engineering. This enables fabrication of cell-laden hydrogels with precise control over the architecture of the scaffold and the location of cells, growth factors, and other biological cues of interest. We first discuss the challenges that exist in terms of choosing a bioprinter, ensuring mechanical support and printability of a material, and minimizing cellular stress for cell-laden prints. We then explain the different crosslinking methods commonly used in hydrogel printing and approaches to alter bioink crosslinking mechanisms. We discuss material selection for bioprinting with elaboration on common materials that have been used and a review of multi-material prints involving hydrogels. We also explore the use of a single, mixed bioink to fabricate complex but homogeneous constructs and multiple, independent bioinks to fabricate complex heterogeneous tissue constructs within the multi-material review. We conclude with a summary of the current state of the field and an outlook on future research.

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Acknowledgments

This work was supported by a seed grant from the Sheikh Zayed Institute for Pediatric Surgical Innovation at Children’s National Medical Center and the A. James Clark School of Engineering at the University of Maryland. Additional funding was provided by the National Science Foundation/US Food and Drug Administration Scholar in Residence Program (CBET1445700). The content is solely the responsibility of the authors and does not necessarily represent the official views of these funding sources.

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Authors and Affiliations

  1. Fischell Department of Bioengineering, University of Maryland, Jeong H. Kim Engineering Building, College Park, MD, 20742, USA

    Navein Arumugasaamy, Hannah B. Baker & John P. Fisher

  2. Sheikh Zayed Institute for Pediatric Surgical Innovation, Children’s National Health System, Washington, DC, 20010, USA

    Navein Arumugasaamy, Peter C. W. Kim & John P. Fisher

  3. Center for Devices & Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA

    Hannah B. Baker & David S. Kaplan

  4. School of Medicine and Health Sciences, The George Washington University, Washington, DC, 20052, USA

    Peter C. W. Kim

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  1. Navein Arumugasaamy
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  2. Hannah B. Baker
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  3. David S. Kaplan
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  4. Peter C. W. Kim
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  5. John P. Fisher
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Correspondence to John P. Fisher .

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Editors and Affiliations

  1. Group Inst. of Materials Science a. Techology, Technische Universität Wien Group Inst. of Materials Sci. a. Techn., Wien, Austria

    Aleksandr Ovsianikov

  2. Institute for Regenerative Medicine, Wake Forest School of Medicine Institute for Regenerative Medicine, Winston-Salem, North Carolina, USA

    James Yoo

  3. 3D Bioprinting Solutions (3D Bio) , Moscow, Russia

    Vladimir Mironov

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Arumugasaamy, N., Baker, H.B., Kaplan, D.S., Kim, P.C.W., Fisher, J.P. (2016). Fabrication and Printing of Multi-material Hydrogels. In: Ovsianikov, A., Yoo, J., Mironov, V. (eds) 3D Printing and Biofabrication. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-40498-1_13-1

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  • DOI: https://doi.org/10.1007/978-3-319-40498-1_13-1

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