Skip to main content
SpringerLink
Log in
Book cover

Sensors and Microsystems pp 165–168Cite as

Electrochemical Immunoassay for Mucin 1 Detection as a Diagnostic Tool in Ovarian Cancer

  • Conference paper
  • First Online:

  • 2423 Accesses

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 268))

Abstract

The immunoassay developed for Mucin 1 (MUC1) detection is based on a sandwich format in which a primary antibody immobilized on the surface of magnetic beads specifically binds the MUC1 protein. The sandwich immunoassay is performed by adding secondary anti-MUC1 and an alkaline-phosphatase-labeled third antibody. After, the modified magnetic beads are captured by a magnet on the surface of a graphite working electrode, and the electrochemical detection is thus achieved through the addition of 1-naphthyl phosphate. 1-naphthol, produced during the enzymatic reaction, is detected using differential pulse voltammetry (DPV). The performance of the assay in terms of sensitivity, reproducibility, and selectivity has been studied. MUC1 detection is performed in buffered solutions and serum sample.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  1. M. Malvezzi, P. Bertuccio, F. Levi, C. La Vecchia, E. Negri. European cancer mortality predictions for the year 2013. Ann. Oncol. 24, 792-800 (2013).

    Article  Google Scholar 

  2. http://eco.iarc.fr/ (last accessed on 1st May 2013).

  3. J. J. Ott, A. Ullrich, A. B. Miller. The importance of early symptom recognition in the context of early detection and cancer survival. European Journal of Cancer 45, 2743-2748 (2009).

    Article  Google Scholar 

  4. A. Ravalli, G. P. dos Santos, M. Ferroni, G. Faglia, H. Yamanaka,G. Marrazza. New label free CA125 detection based on gold nanostructured screen-printed electrode. Sensors and Actuators B 179, 194-200 (2013).

    Article  Google Scholar 

  5. Z. Taleat, A. Ravalli, M. Mazloum-Ardakani, G. Marrazza. CA 125 immunosensor based on poly-anthranilic acid modified screen-printed electrodes. Electroanalysis 25, 269-277 (2013).

    Article  Google Scholar 

  6. I. E. Tothill. Biosensors for cancer markers diagnosis. Seminars in Cell & Developmental Biology 20, 55-62 (2009).

    Article  Google Scholar 

  7. M. Brayman, A. Thathiah, D. D. Carson. MUC1: a multifunctional cell surface component of reproductive tissue epithelia. Reprod. Biol. Endocrinol. 2, 4 (2004).

    Article  Google Scholar 

  8. A. Zani, S. Laschi, M. Mascini, G. Marrazza. A New Electrochemical Multiplexed Assay for PSA Cancer Marker Detection. Electroanalysis, 23, 91-99 (2011).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy

    Andrea Ravalli & Giovanna Marrazza

  2. Department of Analytical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, “Iuliu Hatieganu”, Pasteur 4, Cluj-Napoca, Romania

    Anca Florea, Cecilia Cristea & Robert Sandulescu

Authors
  1. Andrea Ravalli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Giovanna Marrazza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anca Florea
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cecilia Cristea
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Robert Sandulescu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Giovanna Marrazza .

Editor information

Editors and Affiliations

  1. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  2. Department of Information Engineering, University of Brescia, Brescia, Italy

    Vittorio Ferrari

  3. SENSOR Laboratory, IDASC, National Research Council (CNR), Brescia, Italy

    Andrea Ponzoni

  4. Department of Information Engineering, University of Brescia, Brescia, Italy

    Giorgio Sberveglieri

  5. Department of Information Engineering, University of Brescia, Brescia, Italy

    Marco Ferrari

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer International Publishing Switzerland

About this paper

Cite this paper

Ravalli, A., Marrazza, G., Florea, A., Cristea, C., Sandulescu, R. (2014). Electrochemical Immunoassay for Mucin 1 Detection as a Diagnostic Tool in Ovarian Cancer. In: Di Natale, C., Ferrari, V., Ponzoni, A., Sberveglieri, G., Ferrari, M. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 268. Springer, Cham. https://doi.org/10.1007/978-3-319-00684-0_31

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-00684-0_31

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-00683-3

  • Online ISBN: 978-3-319-00684-0

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation