Abstract
A large number of novel synthetic compounds representing smaller parts of original peptidoglycan molecules have been synthesized and found to possess versatile biological activity, particularly immunomodulating properties. A series of compounds containing the adamantyl residues coupled to peptides and glycopeptides characteristic for bacterial peptidoglycan was described. The new adamantylpeptides and adamantylglycopeptides were prepared starting from N-protected racemic adamantylglycine and dipeptide l-Ala-d-isoglutamine. The adamantyl glycopeptides were obtained by coupling the adamantyltripeptides with alpha-d-mannose moiety through spacer molecule of fixed chirality. Since the starting material was d,l-(adamantyl-glycine) the condensation products with the dipeptide were mixtures of diastereoisomers. The obtained diastereoisomers were separated, characterized, and tested for immunostimulating activity. An HPLC method for purity testing was developed and adapted for the particular compounds.
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Acknowledgments
We thank the Ministry of Science, Education and Sport of the Republic of Croatia for its financial support. Projects No. 021-0212432-2431 and 119-1191344-3121 are acknowledged.
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Frkanec, R., Vranešić, B., Tomić, S. (2013). Adamantoylated Biologically Active Small Peptides and Glycopeptides Structurally Related to the Bacterial Peptidoglycan. In: Cudic, P. (eds) Peptide Modifications to Increase Metabolic Stability and Activity. Methods in Molecular Biology, vol 1081. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-652-8_7
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DOI: https://doi.org/10.1007/978-1-62703-652-8_7
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