Abstract
Transcription factors control gene expression by binding to noncoding regions of DNA known as cis-regulatory elements (CREs; i.e., enhancer/promoters). Traditionally, cis-regulatory analysis has been carried out via mouse transgenesis which is time-consuming and nonquantitative. Electroporation of DNA reporter constructs into living mouse tissue is a rapid and effective alternative to transgenesis which permits quantitative assessment of cis-regulatory activity. Here, we present a simple technique for quantifying the activity of photoreceptor-specific CREs in living explanted mouse retinas.
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Montana, C.L., Myers, C.A., Corbo, J.C. (2012). Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_23
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DOI: https://doi.org/10.1007/978-1-62703-080-9_23
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Publisher Name: Humana Press, Totowa, NJ
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