Abstract
Long-term in vitro cultures of blood-stage parasites are so far feasible only for Plasmodium falciparum and P. knowlesi. In this chapter, we describe short-term ex vivo culturing of P. cynomolgi and P. vivax. We also describe long-term in vitro culturing of P. knowlesi as well as some techniques for synchronizing parasites. Cultured parasites can be used for a variety of purposes, e.g., for in vitro drug assays and antibody-mediated growth inhibition assays.
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Acknowledgments
The authors wish to thank Hasting Ozwara Suba for setting up the long-term in vitro culture of P. knowlesi, Joyce Verburgh for help in the in vitro drug assays, Els Klooster for providing parasite pictures, and Henk van Westbroek for designing the figures.
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Zeeman, AM., der Wel, A.Vv., Kocken, C.H.M. (2012). Ex Vivo Culture of Plasmodium Vivax and Plasmodium Cynomolgi and In Vitro Culture of Plasmodium Knowlesi Blood Stages. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_4
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DOI: https://doi.org/10.1007/978-1-62703-026-7_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-025-0
Online ISBN: 978-1-62703-026-7
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