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Uniparental Embryos in the Study of Genomic Imprinting

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Genomic Imprinting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 925))

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Abstract

Nuclear transplantation has been used to study genomic imprinting. Available nuclear transfer methods include pronuclear transfer (PNT), intracytoplasmic sperm injection, and round spermatid injection. By generating uniparental embryos that have exclusively paternal or maternal genomes, it is possible to study the functions of the parental genomes separately. It is possible to compare functions in haploid and diploid states. In addition, nuclear transfer allows the effects of the ooplasm, including mitochondria, to be distinguished from effects of the maternally inherited chromosomes. PNTs can also be used to study epigenetic modifications of the parental genomes by the ooplasm. This chapter reviews the methods employed to generate uniparental embryonic constructs for these purposes.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, The Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, USA

    Yong Cheng & Dasari Amarnath

  2. Department of Biochemistry, The Fels Institute for Cancer Research & Molecular Biology, Temple University School of Medicine, Philadelphia, PA, USA

    Keith E. Latham

Authors
  1. Yong Cheng
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  2. Dasari Amarnath
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  3. Keith E. Latham
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Corresponding author

Correspondence to Keith E. Latham .

Editor information

Editors and Affiliations

  1. School of Medicine, Fels Institute, Temple University, N. Broad Street 3307, Philadelphia, 19140, Pennsylvania, USA

    Nora Engel

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Cheng, Y., Amarnath, D., Latham, K.E. (2012). Uniparental Embryos in the Study of Genomic Imprinting. In: Engel, N. (eds) Genomic Imprinting. Methods in Molecular Biology, vol 925. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-011-3_1

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  • DOI: https://doi.org/10.1007/978-1-62703-011-3_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-010-6

  • Online ISBN: 978-1-62703-011-3

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