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Astrocytes

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Richard Milner

    1. Dept. Molecular & Experimental, Scripps Research Institute, La Jolla, USA

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  • Provides a selection of the key cellular,molecular, and biochemical techniques that are used in studying the many and varied functions of this fascinating cell
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 814)

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Table of contents (37 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overview of Astrocyte Biology

    1. Front Matter

      Pages 1-1
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    2. Astrocytes: Multitalented Stars of the Central Nervous System

      • Bruce R. Ransom, Christopher B. Ransom
      Pages 3-7

    3. Neurogenic Astrocytes and Their Glycoconjugates: Not Just “Glue” Anymore

      • Dennis A. Steindler
      Pages 9-22

    4. Heterogeneity of Astrocytic Form and Function

      • Nancy Ann Oberheim, Steven A. Goldman, Maiken Nedergaard
      Pages 23-45

  3. Isolation and Culture of Astrocytes

    1. Front Matter

      Pages 47-47
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    2. Preparation of Mixed Glial Cultures from Postnatal Rat Brain

      • Jean de Vellis, Ruth Cole
      Pages 49-59

    3. Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia

      • Tracy F. Uliasz, Mary E. Hamby, Nicole A. Jackman, James A. Hewett, Sandra J. Hewett
      Pages 61-79

    4. Derivation of Microglia-Free Astrocyte Cultures from Neural Stem Cells

      • Jennifer V. Welser, Richard Milner
      Pages 81-91

    5. Isolation and Culture of Spinal Cord Astrocytes

      • Amber E. Kerstetter, Robert H. Miller
      Pages 93-104

    6. Isolation and Culture of Astrocytes from the Retina and Optic Nerve

      • Thomas J. Lukas, Ai Ling Wang
      Pages 105-115

    7. Primary Cultures of Astrocytes from Fetal Bovine Brain

      • Cristina Ballarin, Antonella Peruffo
      Pages 117-126

    8. Isolation and Cultivation of Porcine Astrocytes

      • Dennis J. Bobilya
      Pages 127-135

    9. Isolation and Culture of Human Astrocytes

      • Ariane Sharif, Vincent Prevot
      Pages 137-151

    10. Cell Culturing of Caenorhabditis elegans Glial Cells for the Assessment of Cytosolic Ca2+ Dynamics

      • Randy F. Stout Jr., Vladimir Parpura
      Pages 153-174

  4. Analysis of Astrocyte Functions In Vitro

    1. Front Matter

      Pages 175-175
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    2. Astrocyte Polarization and Wound Healing in Culture: Studying Cell Adhesion Molecules

      • H. Peng, S. Carbonetto
      Pages 177-188

    3. An In Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity

      • Ina-Beate Wanner
      Pages 189-219

    4. Use of Gel Zymography to Examine Matrix Metalloproteinase (Gelatinase) Expression in Brain Tissue or in Primary Glial Cultures

      • Harald Frankowski, Yu-Huan Gu, Ji Hoe Heo, Richard Milner, Gregory J. del Zoppo
      Pages 221-233

    5. ELISA Methodology to Quantify Astrocyte Production of Cytokines/Chemokines In Vitro

      • Hongwei Qin, Etty N. Benveniste
      Pages 235-249
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Keywords

About this book

Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma.  Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS, including generation of neural stem cells, synaptogenesis, and regulation of the blood-brain barrier and neurovascular unit. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • Dept. Molecular & Experimental, Scripps Research Institute, La Jolla, USA

    Richard Milner

Bibliographic Information

  • Book Title: Astrocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Richard Milner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-452-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-451-3Published: 06 December 2011

  • Softcover ISBN: 978-1-4939-5859-7Published: 23 August 2016

  • eBook ISBN: 978-1-61779-452-0Published: 07 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 575

  • Topics: Neurosciences, Human Physiology

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