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Recombinant Gene Expression pp 629–641Cite as

Recombinant Protein Expression in Milk of Livestock Species

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 824))

Abstract

Producing complex recombinant proteins in the milk of transgenic animals offers several advantages: large amounts of proteins can be obtained, and in most cases, these proteins are properly folded, assembled, cleaved, and glycosylated. The level of expression of foreign genes in the mammalian gland cannot be predicted in all cases, and appropriate vectors must be used. The main elements of these vectors are as follows: a well-characterized specific promoter, the coding region of the gene of interest, preferably with a homologous or heterologous intron, to improve transcription efficiency, and an insulator or boundary element to counteract the chromosomal position effects at the integration site. Once high expression levels are achieved, and the recombinant protein is purified, an essential step in the analysis of the final product is determining its degree of glycosylation. This is an important readout because it can affect among other parameters the stability and immunogenicity of the recombinant protein.

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Acknowledgments

This work was supported by the grant OM-00118/2008.

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Authors and Affiliations

  1. Agricultural Biotechnology Center, Gödöll, Hungary

    Zsuzsanna Bösze & László Hiripi

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  1. Zsuzsanna Bösze
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  2. László Hiripi
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Correspondence to Zsuzsanna Bösze .

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  1. Dept. Chemistry & Physics, Arkansas State University, Jonesboro, University Loop E. 504, State University, 72401, Arkansas, USA

    Argelia Lorence

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Bösze, Z., Hiripi, L. (2012). Recombinant Protein Expression in Milk of Livestock Species. In: Lorence, A. (eds) Recombinant Gene Expression. Methods in Molecular Biology, vol 824. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-433-9_34

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  • DOI: https://doi.org/10.1007/978-1-61779-433-9_34

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  • Publisher Name: Humana Press, Totowa, NJ

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