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  • © 2011

Gene Expression Profiling

Methods and Protocols

  • Presents a broad range of available and developing techniques for the analysis of gene expression at the DNA, RNA, and protein levels
  • Features easy to follow, step-by-step procedures, ready for the lab
  • Includes vital tips and expert implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 784)

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Softcover Book USD 79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
  2. Real-Time PCR and Multiplex Approaches

    • Olga L. Gurvich, Mikhail Skoblov
    Pages 1-13
  3. Software and Tools for Microarray Data Analysis

    • Jai Prakash Mehta, Sweta Rani
    Pages 41-53
  4. Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumour Cells

    • Anne M. Friel, John Crown, Lorraine O’Driscoll
    Pages 55-75
  5. Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases

    • Serena Germano, Lorraine O’Driscoll
    Pages 109-121
  6. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis

    • Kishore Reddy Katikireddy, Finbarr O’Sullivan
    Pages 155-167
  7. Advanced Microscopy: Laser Scanning Confocal Microscopy

    • Orla Hanrahan, James Harris, Chris Egan
    Pages 169-180
  8. Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling

    • Sweta Rani, Keith O’Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek W. Radomski et al.
    Pages 181-195
  9. Back Matter

    Pages 209-211

About this book

Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.  In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.

Reviews

From the reviews of the second edition:

“This beautifully illustrated book on gene expression profiling provides detailed protocols for a broad range of techniques … for the analysis of gene expression at the DNA, RNA, and protein levels. … Although written for researchers, the book will be helpful for graduate students and others interested in learning about gene expression. … This is a valuable resource on methods and protocols necessary to understand the changes in gene expression in normal and pathological states in pursuit of better treatments.” (Omer Iqbal, Doody’s Book Reviews, April, 2012)

Editors and Affiliations

  • School of Pharmacy, and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland

    Lorraine O'Driscoll

Bibliographic Information

  • Book Title: Gene Expression Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Lorraine O'Driscoll

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-289-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-288-5Published: 07 September 2011

  • Softcover ISBN: 978-1-4939-6173-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-289-2Published: 08 September 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 211

  • Number of Illustrations: 22 b/w illustrations, 15 illustrations in colour

  • Topics: Human Genetics, Gene Expression

Buy it now

Buying options

Softcover Book USD 79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access