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Proprotein Convertases pp 191–206Cite as

The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 768))

Abstract

Vascular smooth muscle cell (VSMC) proliferation and migration represent key features in atherosclerosis and restenosis. The proprotein convertases (PCs) furin and PC5 are highly expressed in human atheroma and are putatively involved in vascular lesion formation via the activation of precursor proteins, essential for cell proliferation and migration. In vitro assays have identified these PCs to govern cell functions via endoproteolytic cleavage of key substrates, including pro-integrins and pro-matrix metalloproteinases. In vivo gene expression studies of furin/PC5 and their substrates demonstrate their coordinated regulation in animal models of vascular remodelling and in human atherosclerotic lesions. Here we describe in vitro and in vivo models to investigate the function of furin/PC5 in VSMCs and in vascular lesion formation. In conjunction with the development of novel PC inhibitors, this should facilitate the development of new strategies targeting PCs in cardiovascular disease.

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Author information

Authors and Affiliations

  1. Department of Medicine/Cardiology, Deutsches Herzzentrum Berlin, D-13353, Berlin, Germany

    Philipp Stawowy

  2. Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, and Center for Cardiovascular Research (CCR), Charité – University Medicine Berlin, D-13353, Berlin, Germany

    Kai Kappert

Authors
  1. Philipp Stawowy
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  2. Kai Kappert
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Corresponding author

Correspondence to Philipp Stawowy .

Editor information

Editors and Affiliations

  1. Chronic Disease Program, Ottawa Hospital Research Institute, Parkdale Avenue 725, Ottawa, K1Y 4E9, Ontario, Canada

    Majambu Mbikay

  2. (ICRM), Lab. Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Avenue des Pins Ouest 110, Montreal, H2W 1R7, Québec, Canada

    Nabil G. Seidah

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Stawowy, P., Kappert, K. (2011). The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling. In: Mbikay, M., Seidah, N. (eds) Proprotein Convertases. Methods in Molecular Biology, vol 768. Humana Press. https://doi.org/10.1007/978-1-61779-204-5_9

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  • DOI: https://doi.org/10.1007/978-1-61779-204-5_9

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-203-8

  • Online ISBN: 978-1-61779-204-5

  • eBook Packages: Springer Protocols

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