Abstract
In order to execute their immune functions, leukocytes interact with a broad range of cell types through cell surface receptors, such as those of the immunoglobulin and C-type lectin families, or indirectly through soluble factors. The characterization of activating and inhibitory counterparts of NK cell receptors on myeloid cells, as well as the identification of their physiological ligands, has provided new insights into the underlying mechanisms of immunity and homeostasis. Here, we describe methodology that can be employed to screen for endogenous ligands of type-II C-type lectin-like receptors using reporter cells and Fc fusion proteins.
Key words
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- CRD:
-
Carbohydrate recognition domain
- CTLD:
-
C-Type lectin-like domain
- CTLR:
-
C-Type lectin-like receptor
- MICL:
-
Myeloid inhibitory C-type lectin
- NKC:
-
Natural killer complex
- NKCL:
-
NK-like C-type lectin receptor
- PAMPs:
-
Pathogen-associated molecule patterns
- PRRs:
-
Pattern recognition receptors
- X-Gal:
-
5-Bromo-4-chloro-3-indolyl-beta-d-galactopyranoside
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Acknowledgments
This work was supported by the Wellcome Trust and South African National Research Foundation.
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Pyż, E., Brown, G.D. (2011). Screening for Ligands of C-Type Lectin-Like Receptors. In: Rast, J., Booth, J. (eds) Immune Receptors. Methods in Molecular Biology, vol 748. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-139-0_1
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DOI: https://doi.org/10.1007/978-1-61779-139-0_1
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