Abstract
In order to execute their immune functions, leukocytes interact with a broad range of cell types through cell surface receptors, such as those of the immunoglobulin and C-type lectin families, or indirectly through soluble factors. The characterization of activating and inhibitory counterparts of NK cell receptors on myeloid cells, as well as the identification of their physiological ligands, has provided new insights into the underlying mechanisms of immunity and homeostasis. Here, we describe methodology that can be employed to screen for endogenous ligands of type-II C-type lectin-like receptors using reporter cells and Fc fusion proteins.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- CRD:
-
Carbohydrate recognition domain
- CTLD:
-
C-Type lectin-like domain
- CTLR:
-
C-Type lectin-like receptor
- MICL:
-
Myeloid inhibitory C-type lectin
- NKC:
-
Natural killer complex
- NKCL:
-
NK-like C-type lectin receptor
- PAMPs:
-
Pathogen-associated molecule patterns
- PRRs:
-
Pattern recognition receptors
- X-Gal:
-
5-Bromo-4-chloro-3-indolyl-beta-d-galactopyranoside
References
Sobanov, Y., Bernreiter, A., Derdak, S., Mechtcheriakova, D., Schweighofer, B., Duchler, M., Kalthoff, F., and Hofer, E. (2001) A novel cluster of lectin-like receptor genes expressed in monocytic, dendritic and endothelial cells maps close to the NK receptor genes in the human NK gene complex, Eur J Immunol 31, 3493–3503.
Pyz, E., Marshall, A. S., Gordon, S., and Brown, G. D. (2006) C-type lectin-like receptors on myeloid cells, Ann Med 38, 242–251.
Huysamen, C., and Brown, G. D. (2009) The fungal pattern recognition receptor, Dectin-1, and the associated cluster of C-type lectin-like receptors, FEMS Microbiol Lett 290, 121–128.
Yokoyama, W. M., and Plougastel, B. F. (2003) Immune functions encoded by the natural killer gene complex, Nat Rev Immunol 3, 304–316.
Drickamer, K., and Fadden, A. J. (2002) Genomic analysis of C-type lectins, Biochem Soc Symp 69, 59–72.
Kerrigan, A. M., Dennehy, K. M., Mourao-Sa, D., Faro-Trindade, I., Willment, J. A., Taylor, P. R., Eble, J. A., Reis e Sousa, C., and Brown, G. D. (2009) CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils, J Immunol 182, 4150–4157.
Robinson, M. J., Sancho, D., Slack, E. C., LeibundGut-Landmann, S., and Reis e Sousa, C. (2006) Myeloid C-type lectins in innate immunity, Nat Immunol 7, 1258–1265.
Pyz, E., Huysamen, C., Marshall, A. S., Gordon, S., Taylor, P. R., and Brown, G. D. (2008) Characterisation of murine MICL (CLEC12A) and evidence for an endogenous ligand, Eur J Immunol 38, 1157–1163.
Brown, G. D., Taylor, P. R., Reid, D. M., Willment, J. A., Williams, D. L., Martinez-Pomares, L., Wong, S. Y., and Gordon, S. (2002) Dectin-1 is a major beta-glucan receptor on macrophages, J Exp Med 196, 407–412.
Smith, H. R., Heusel, J. W., Mehta, I. K., Kim, S., Dorner, B. G., Naidenko, O. V., Iizuka, K., Furukawa, H., Beckman, D. L., Pingel, J. T., Scalzo, A. A., Fremont, D. H., and Yokoyama, W. M. (2002) Recognition of a virus-encoded ligand by a natural killer cell activation receptor, Proc Natl Acad Sci USA 99, 8826–8831.
Iizuka, K., Naidenko, O. V., Plougastel, B. F., Fremont, D. H., and Yokoyama, W. M. (2003) Genetically linked C-type lectin-related ligands for the NKRP1 family of natural killer cell receptors, Nat Immunol 4, 801–807.
Carlyle, J. R., Jamieson, A. M., Gasser, S., Clingan, C. S., Arase, H., and Raulet, D. H. (2004) Missing self-recognition of Ocil/Clr-b by inhibitory NKR-P1 natural killer cell receptors, Proc Natl Acad Sci USA 101, 3527–3532.
Rosen, D. B., Bettadapura, J., Alsharifi, M., Mathew, P. A., Warren, H. S., and Lanier, L. L. (2005) Cutting edge: lectin-like transcript-1 is a ligand for the inhibitory human NKR-P1A receptor, J Immunol 175, 7796–7799.
Graham, L. M., Tsoni, S. V., Willment, J. A., Williams, D. L., Taylor, P. R., Gordon, S., Dennehy, K., and Brown, G. D. (2006) Soluble Dectin-1 as a tool to detect beta-glucans, J Immunol Methods 314, 164–169.
Taylor, P. R., Brown, G. D., Geldhof, A. B., Martinez-Pomares, L., and Gordon, S. (2003) Pattern recognition receptors and differentiation antigens define murine myeloid cell heterogeneity ex vivo, Eur J Immunol 33, 2090–2097.
Lutz, M. B., Kukutsch, N., Ogilvie, A. L., Rossner, S., Koch, F., Romani, N., and Schuler, G. (1999) An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow, J Immunol Methods 223, 77–92.
Suzuki-Inoue, K., Kato, Y., Inoue, O., Kaneko, M. K., Mishima, K., Yatomi, Y., Yamazaki, Y., Narimatsu, H., and Ozaki, Y. (2007) Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells, J Biol Chem 282, 25993–26001.
Acknowledgments
This work was supported by the Wellcome Trust and South African National Research Foundation.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Pyż, E., Brown, G.D. (2011). Screening for Ligands of C-Type Lectin-Like Receptors. In: Rast, J., Booth, J. (eds) Immune Receptors. Methods in Molecular Biology, vol 748. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-139-0_1
Download citation
DOI: https://doi.org/10.1007/978-1-61779-139-0_1
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-138-3
Online ISBN: 978-1-61779-139-0
eBook Packages: Springer Protocols