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Acquisition, Preparation, and Functional Assessment of Human NK Cells for Adoptive Immunotherapy

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Immunotherapy of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 651))

Abstract

Human natural killer (NK) cells, a subset of peripheral blood lymphocytes that lack a T- or B-cell receptor, play a crucial role in the innate immune response to viruses and malignant cells. NK cells differentiate infected or malignant cells from normal cells by a complex balance between activating and inhibitory receptor–ligand interactions. Unlike T cells, NK cells do not proliferate in vitro in response to simple crosslinking of a single activating receptor. While many methods to study T-cell function and phenotype can also be applied to NK cells, this chapter addresses methods that are unique to the preparation and assessment of human NK cells for immunotherapy.

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Author information

Authors and Affiliations

  1. Hematology/Oncology Section and Division of Blood and Marrow Transplant, University of Minnesota, Minneapolis, MN, USA

    Michael R. Verneris

  2. Laboratory Research, Department of Oncology, St. Jude Children’s Research Hospital, Memphis, TN, USA

    Dario Campana

Authors
  1. Dean A. Lee
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  2. Michael R. Verneris
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  3. Dario Campana
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Editor information

Editors and Affiliations

  1. , Center for Cell and Gene Therapy, Baylor College of Medicine, Baylor Plaza One, Houston, 77030, USA

    Patricia Yotnda

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Lee, D.A., Verneris, M.R., Campana, D. (2010). Acquisition, Preparation, and Functional Assessment of Human NK Cells for Adoptive Immunotherapy. In: Yotnda, P. (eds) Immunotherapy of Cancer. Methods in Molecular Biology, vol 651. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-786-0_4

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  • DOI: https://doi.org/10.1007/978-1-60761-786-0_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-785-3

  • Online ISBN: 978-1-60761-786-0

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