Protocols for Adult Stem Cells

1. Front Matter

   Pages i-x

   PDF

2. In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells

   • Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca, Dawne N. Shelton, Bryan E. Welm, Mary Helen Barcellos-Hoff, Carlos Ortiz-de-Solorzano

   Pages 1-28

3. Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies

   • Irineu Illa-Bochaca, Rodrigo Fernandez-Gonzalez, Dawne N. Shelton, Bryan E. Welm, Carlos Ortiz-de-Solorzano, Mary Helen Barcellos-Hoff

   Pages 29-47

4. Use of Stem Cell Markers in Dissociated Mammary Populations

   • Dawne N. Shelton, Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca, Carlos Ortiz-de-Solorzano, Mary Helen Barcellos-Hoff, Bryan E. Welm

   Pages 49-55

5. Isolation of Adult Hippocampal Neural Progenitors

   • Joseph Peltier, Brandi K. Ormerod, David V. Schaffer

   Pages 57-63

6. In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors

   • Joseph Peltier, Smita Agrawal, Matthew J. Robertson, David V. Schaffer

   Pages 65-87

7. In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors

   • Matthew J. Robertson, Joseph Peltier, David V. Schaffer

   Pages 89-102

8. Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors

   • Joseph Peltier, David V. Schaffer

   Pages 103-116

9. Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix

   • Jennifer S. Park, Craig Hashi, Song Li

   Pages 117-137

10. Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis

    • Steve Khachi, Yiqian Zhu, Guo-Yuan Yang

    Pages 139-148

11. Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells

    • Michael J. Conboy, Irina M. Conboy

    Pages 149-163

12. Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells

    • Michael J. Conboy, Massimiliano Cerletti, Amy J. Wagers, Irina M. Conboy

    Pages 165-173

13. Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy

    • Anshu Agrawal

    Pages 175-183

14. Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of In Vivo Muscle

    • Tathagata Chaudhuri, Florian Rehfeldt, H. Lee Sweeney, Dennis E. Discher

    Pages 185-202

15. Back Matter

    Pages 203-207

    PDF

Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body’s natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.

• Endothelial
• Mammalian
• Mammary
• Mesenchymal
• Muscle
• Nerve
• Vivo
• cell biology
• molecular biology
• myogenesis
• Cell culture

From the reviews:

“This volume in the popular Methods in Molecular Biology series provides a snapshot of research techniques and protocols for identifying and culturing these amazing tissue-specific stem cells. … The book is written for basic science and clinical researchers interested in stem cell biology, as well as mechanisms of carcinogenesis and malignancy. It also will be valued by biomedical scientists and clinicians interested in cytotechnology and biotechnology. … The topic is fascinating and clinically relevant. I enjoyed learning about antigen-priming dendritic cells.” (Bruce A. Fenderson, Doody’s Review Service, August, 2010)

• Dept. Bioengineering, University of California, Berkeley, Berkeley, USA

  Irina M. Conboy, Song Li

• Helen Wills Neuroscience Inst., University of California, Berkeley, Berkeley, USA

  David V. Schaffer

• Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Berkeley, USA

  Mary Helen Barcellos-Hoff

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