Skip to main content
SpringerLink
Log in
Book cover

Matrix Metalloproteinase Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Ian M. Clark

    1. School of Biological Sciences, University of East Anglia, Norwich, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes two comprehensive review chapters and twenty-five detailed method chapters
  • Provides state-of-the-art methodologies
  • Covers expression, purification, detection, and assay of MMPs and TIMPs
  • Details many essential techniques in one easily accessible volume
  • Updated and improved second edition
  • Chapters include the 'tricks of the trade' written by leading researchers
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 622)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. MMPs and TIMPs: An Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Metalloproteases and the Degradome

      • Alejandro P. Ugalde, Gonzalo R. Ordóñez, Pedro M. Quirós, Xose S. Puente, Carlos López-Otín
      Pages 3-29

    3. Mouse Models of MMP and TIMP Function

      • Sean E. Gill, Sean Y. Kassim, Timothy P. Birkland, William C. Parks
      Pages 31-52

  3. Expression and Purification of MMPs and TIMPs

    1. Front Matter

      Pages 53-53
      Download chapter PDF

    2. Expression of Recombinant MMP-28 in Mammalian Cells

      • Ursula R. Rodgers, Ian M. Clark
      Pages 55-65

    3. Expression of Recombinant Matrix Metalloproteinases in Escherichia coli

      • L. Jack Windsor, Darin L. Steele
      Pages 67-81

    4. Expression of Recombinant ADAMTS in Insect Cells

      • Gavin C. Jones, Mireille N. Vankemmelbeke, David J. Buttle
      Pages 83-98

    5. Expression and Purification of Membrane-Type MMPs

      • Jing Nie, Duanqing Pei
      Pages 99-110

    6. Refolding of TIMP-2 from Escherichia coli Inclusion Bodies

      • Richard A. Williamson
      Pages 111-121

    7. Purification of MMPs and TIMPs

      • Ken-ichi Shimokawa, Hideaki Nagase
      Pages 123-155

  4. Detection of MMPs and TIMPs

    1. Front Matter

      Pages 157-157
      Download chapter PDF

    2. Real-Time PCR Expression Profiling of MMPs and TIMPs

      • Caroline J. Pennington, Dylan R. Edwards
      Pages 159-173

    3. Analysis of the Degradome with the CLIP-CHIPâ„¢ Microarray

      • Reinhild Kappelhoff, Ulrich auf dem Keller, Christopher M. Overall
      Pages 175-193

    4. In Situ Hybridization for Metalloproteinases and Their Inhibitors

      • Tiina L. Hurskainen, Suneel S. Apte
      Pages 195-209

    5. Immunohistochemistry of MMPs and TIMPs

      • Yasunori Okada
      Pages 211-219

    6. Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5ʹ Nuclease Assay

      • Ross Laxton, Shu Ye
      Pages 221-229

  5. Assay of MMP and TIMP Activities

    1. Front Matter

      Pages 231-231
      Download chapter PDF

    2. Methods for Studying Activation of Matrix Metalloproteinases

      • Vera Knäuper, Gillian Murphy
      Pages 233-243

    3. Assay of Matrix Metalloproteinases Against Matrix Substrates

      • Tim E. Cawston, Rachel L. Lakey, Andrew D. Rowan
      Pages 245-255
Back to top

Keywords

About this book

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms.  Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field.  Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Reviews

Review of the first edition: "The editor, Ian M. Clark, has done an excellent job in putting together a collection of articles that span what is now a fairly wide field. ...the content and writing are of a high standard...In chapter 3, covering structural studies and contributed by Wolfram Bode and Klaus Maskos, the authors point out the absence of specific discussions of three-dimensional structures amongst the plethora of general reviews on MMPs and TIMPs. Their contribution expertly fills that void....Indeed the 'Notes' section, at the end of each chapter, is where the benefit of much, often hard-earned, experience is to be reaped and is a valuable addition in almost every case. The last two sections, between them, cover detection of MMp and TIMP expression at both RNA and protein levels, and actively assays. Hore, particular mention must be made of the chapter dealing with zymography and reverse zymography in which Susan Hawkes and coauthors have carefully explained this technique. Their use of figures with detailed interpretation is really excellent and alone could justify the purchase of this book for many labs....this book is recommended as a reference text for all labs engaged in MMP research. There is enough variety for established research labs to find some new methods, and, for newcomers to the field, it should make working with MMPs and their inhibitors a very straightforward prospect." - TRENDS in Cell Biology

Editors and Affiliations

  • School of Biological Sciences, University of East Anglia, Norwich, United Kingdom

    Ian M. Clark

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation