Affinity Chromatography

1. Front Matter

   Pages i-x

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2. An Overview of Affinity Chromatography

   • Meir Wilchek, Irwin Chaiken

   Pages 1-6

3. Weak Affinity Chromatography

   • Magnus Strandh, HÅkan S. Andersson, Sten Ohlson

   Pages 7-23

4. Fluidized-Bed Receptor-Affinity Chromatography

   • Cheryl L. Spence, Pascal Bailon

   Pages 25-39

5. Site-Specific Immobilization of Antibodies to Protein G-Derivatized Solid Supports

   • Frank J. Podlaski, Alvin S. Stern

   Pages 41-48

6. Affinity Purification of Monoclonal Antibodies

   • Alexander Schwarz

   Pages 49-56

7. Protein A Mimetic (PAM) Affinity Chromatography

   • Giorgio Fassina

   Pages 57-68

8. Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography

   • David S. Hage

   Pages 69-82

9. Mini-Antibody Affinity Chromatography of Lysozyme

   • Gjalt W. Welling, Sytske Welling-Wester

   Pages 83-88

10. Nitrocellulose-Based Immunoaffinity Chromatography

    • Joseph Thalhamer, Peter Hammerl, Arnulf Hartl

    Pages 89-93

11. Immunoaffinity Chromatography

    • Anuradha Subramanian

    Pages 95-104

12. Affinity Partitioning of Proteins

    • Göte Johansson

    Pages 105-117

13. Boronate Affinity Chromatography

    • Xiao-Chuan Liu, William H. Scouten

    Pages 119-128

14. Dye-Ligand Affinity Chromatography for Protein Separation and Purification

    • Nikolaos E. Labrou

    Pages 129-139

15. DNA Affinity Chromatography

    • Priya Sethu Chockalingam, Luis A. Jurado, F. Darlene, Harry W. Jarrett

    Pages 141-153

16. Affinity Chromatography of Pyrogens

    • Satoshi Minobe, Takeji Shibatani, Tetsuya Tosa

    Pages 155-162

17. Western Cross Blot

    • Peter Hammerl, Arnulf Hartl, Johannes Freund, Joseph Thalhamer

    Pages 163-173

18. Affinity Perfusion Chromatography

    • Neal F. Gordon, Duncan H. Whitney, Tom R. Londo, Tim K. Nadler

    Pages 175-193

19. Phage Display Technology

    • George K. Ehrlich, Wolfgang Berthold, Pascal Bailon

    Pages 195-208

20. Phage Display Technology

    • George K. Ehrlich, Pascal Bailon, Wolfgang Berthold

    Pages 209-220

Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.

• Department of Biopharmaceuticals, Hoffmann-La Roche Inc., Nutley

  Pascal Bailon, George K. Ehrlich, Wen-Jian Fung, Wolfgang Berthold

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