Abstract
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs, which have been utilized for decades, rabbit mAbs are defined reagents with infinite supply, prompting considerable interest in recent years. Phage-display technology based on chimeric rabbit/human Fab libraries allows the selection of rabbit mAbs that cross-react with mouse and human antigens while maintaining high affinity and specificity.
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References
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Acknowledgments
I would like to thank Dr. Carlos F. Barbas III (The Scripps Research Institute, La Jolla, CA) for training me in antibody technologies including phage display, and members of my laboratory at the National Cancer Institute, in particular Drs. Thomas Hofer and Ka Yin Kwong, for their outstanding contributions to further evolving the methodology.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Rader, C. (2009). Generation and Selection of Rabbit Antibody Libraries by Phage Display. In: Dimitrov, A. (eds) Therapeutic Antibodies. Methods in Molecular Biology™, vol 525. Humana Press. https://doi.org/10.1007/978-1-59745-554-1_5
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DOI: https://doi.org/10.1007/978-1-59745-554-1_5
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