Overview
- Contains detailed protocols that describe the use of routine and advanced electrophysiological techniques, such as whole cell patch clamp, perforated patch clamp, excised macropatch, open-cell cell-attached patch clamp, planar patch clamp, and artificial bilayer
- Of major pharmacological interest as a number of chapters describe the use of potassium channel drug screening platforms, planar electrophysiology, and the cardiac safety pharmacology of HERG potassium channels
- Describes optical techniques that allow the real-time study of protein interactions and conformational changes in potassium channels
- Examines both functional experiments and protocols that allow the evaluation of the cellular biology of potassium channels, like transcription, splicing, and protein trafficking
- Features standard laboratory techniques such as cell culture and transfection, oocyte injection, Western blotting, PCR to support these experiments
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 491)
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Table of contents (22 protocols)
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Front Matter
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Methods For Studying Potassium Channel Transcription And Mrna Splicing
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Methods for Studying the Cell Biology of Potassium Channel Proteins
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Electrophysiological Techniques For The Study Of Potassium Channel Function
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Optical Techniques For The Study Of Potassium Channel Function And Interactions
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Methods For Studying Potassium Channelopathies And Pharmacological Modulators
Keywords
About this book
Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.
Reviews
From the reviews:
"This is a very informative book, covering a variety of methods available to study potassium channels … . The protocols are clearly organized and usefully detailed. … Illustrative figures and in some cases, photographs of equipment … adding to the clarity of the methods sections. This book … a valuable reference not only for those already in the potassium channel field but also for studies of other ion channel systems. … each chapter allows bench scientists to undertake new, informed, experimental approaches in their research." (Samantha Miller, Microbiology Today, July, 2009)
“This book is dedicated to experimental methods, which allow us to investigate potassium channels from their genes through gene transcription to protein structure, function and regulation in health and disease. … chapters in this book are written in form of practical laboratory protocols. Therefore the book will be primarily used by practicing scientists and by graduated and post-graduate students for their practical research. Certainly it will find its place in many laboratories investigating various features of potassium channels regulations.” (Ľubica Lacinová, General Physiology and Biophysics, Vol. 29, April, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Potassium Channels
Book Subtitle: Methods and Protocols
Editors: Jonathan D. Lippiat
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-526-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-65-7Published: 23 October 2008
Softcover ISBN: 978-1-61737-940-6Published: 19 November 2010
eBook ISBN: 978-1-59745-526-8Published: 09 November 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 302
Number of Illustrations: 59 b/w illustrations
Topics: Pharmacology/Toxicology, Cell Biology, Membrane Biology