Abstract
Human embryonic stem (ES) cell lines have been derived from the inner cell mass of preimplantation embryos by culturing the cells on mouse embryonic feeder cells (1,2) In these conditions, human ES cells show remarkable proliferative capacity and stability in long-term culture (3) and have the capacity to differentiate into cell types from all three germ layers both in vitro and in vivo (1,2). Therefore, human ES cells may be a source of cells for cell therapies, drug screening, and functional genomics applications.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., et al. (1998) Embryonic stem cell lines derived from human blastocysts, Science 282, 1145–1147.
Reubinoff, B. E., Pera, M. F., Fong, C. Y., Trounson, A., and Bongso, A. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro, Nature Biotechnol. 18, 399–404.
Amit, M., Carpenter, M. K., Inokuma, M. S., et al. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture, Dey. Biol. 227, 271–278.
Smith, A. G., Heath, J. K., Donaldson, D. D., et al. (1988) Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides, Nature 336, 688–690.
Williams, R. L., Hilton, D. J., Pease, S., et al. (1988) Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells, Nature 336, 684–687.
Xu, C., Inokuma, M. S., Denham, J., Golds, K., Kundu, P., Gold, J. D., and Carpenter, M. K. (2001) Feeder-free growth o undifferentiated human embryonic stem cells on defined matrices with conditioned medium, Nature Biotechnol. 19, 971–974.
Robertson, E. J. (1987) Teratocarcinomas and Embryonic Stem Cells: A Practical Approach (Approach, T. a. E. S. c. A. P., ed.), IRL, Washington, DC.
Solter, D. and Knowles, B. B. (1975) Immunosurgery of mouse blastocyst, Proc. Natl. Acad. Sci USA 72, 5099–5102.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media New York
About this chapter
Cite this chapter
Carpenter, M.K., Xu, C., Daigh, C.A., Antosiewicz, J.E., Thomson, J.A. (2003). Protocols for the Isolation and Maintenance of Human Embryonic Stem Cells. In: Chiu, A.Y., Rao, M.S. (eds) Human Embryonic Stem Cells. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-423-8_6
Download citation
DOI: https://doi.org/10.1007/978-1-59259-423-8_6
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61737-484-5
Online ISBN: 978-1-59259-423-8
eBook Packages: Springer Book Archive