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Cell-Based Assays Using iPSCs for Drug Development and Testing pp 175–184Cite as

High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1994))

Abstract

Mitochondrial dysfunction is linked to many neurological diseases; therefore, the ability to measure mitochondrial function is of great use for researching disease and testing potential therapeutics. Here we describe a high-content assay to simultaneously measure mitochondrial membrane potential, morphology and cell viability in iPSC-derived neurons. Neurons are seeded into plates suitable for fluorescent microscopy, stained with the mitochondrial membrane potential-dependent dye TMRM, cytoplasmic dye Calcein AM, and nuclear stain Hoechst 33342. Images are acquired in live cells and analyzed using automated image analysis software.

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Authors and Affiliations

  1. MRC Laboratory for Molecular Cell Biology, University College London, London, UK

    Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler & Michael J. Devine

  2. MRC Laboratory for Molecular Cell Biology, University College London, London, UK

    Paul Gissen

  3. Great Ormond Street Institute of Child Health, University College London, London, UK

    Paul Gissen

  4. NIHR Great Ormond Street Hospital Biomedical Research Centre, University College London, London, UK

    Paul Gissen

Authors
  1. Daniel Little
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  2. Christin Luft
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  3. Olukunbi Mosaku
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  4. Robin Ketteler
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  5. Michael J. Devine
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  6. Paul Gissen
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Corresponding author

Correspondence to Daniel Little .

Editor information

Editors and Affiliations

  1. Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden

    Carl-Fredrik Mandenius

  2. Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK

    James A. Ross

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Little, D., Luft, C., Mosaku, O., Ketteler, R., Devine, M.J., Gissen, P. (2019). High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons. In: Mandenius, CF., Ross, J. (eds) Cell-Based Assays Using iPSCs for Drug Development and Testing. Methods in Molecular Biology, vol 1994. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9477-9_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9477-9_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9476-2

  • Online ISBN: 978-1-4939-9477-9

  • eBook Packages: Springer Protocols

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