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Isolation and Generation of Osteoclasts

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Book cover Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

This chapter describes the isolation, culture, and staining of osteoclasts. The key advantages of this assay are that it allows direct measurement of osteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.

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Acknowledgments

We are grateful to Prof Rodolfo Amprino and Prof Gastone Marotti for introducing us into the bone field, to Prof Pier Carlo Marchisio for the instrumental collaboration in the discovery of the podosomes, and to Prof Steven L. Teitelbaum and Prof Arnold J. Kahn, who believed in our chicken osteoclast isolation method and made it possible to disseminate this knowledge to the bone community.

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Authors and Affiliations

  1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Nadia Rucci & Anna Teti

  2. Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari, Bari, Italy

    Alberta Zallone

Authors
  1. Nadia Rucci
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  2. Alberta Zallone
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  3. Anna Teti
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Corresponding author

Correspondence to Anna Teti .

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Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Rucci, N., Zallone, A., Teti, A. (2019). Isolation and Generation of Osteoclasts. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

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