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Genetic Models of Calpain Deficiency and Ectopic Expression

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Book cover Calpain

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1915))

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Abstract

Genetic manipulation in cell and animal models can provide important insights into gene function and the relationships between gene mutation and disease. This chapter describes methods to generate models of calpain-1 and calpain-2 deficiency, or their recombinant ectopic expression in cultured cells, and to genotype a conditional transgenic mouse model of calpain-1/calpain-2 deficiency that can be used to explore physiologic roles for these calpains.

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References

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Acknowledgment

This work was supported by a grant from the Canadian Institutes of Health Research (81189) to PAG.

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Authors and Affiliations

  1. Department of Pathology and Molecular Medicine, Queen’s University, Kingston, ON, Canada

    Yan Gao, Christine Hall, James MacLeod & Peter A. Greer

  2. Division of Cancer Biology and Genetics, Cancer Research Institute, Kingston, ON, Canada

    Yan Gao, Christine Hall, James MacLeod & Peter A. Greer

Authors
  1. Yan Gao
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  2. Christine Hall
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  3. James MacLeod
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  4. Peter A. Greer
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Corresponding author

Correspondence to Peter A. Greer .

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Editors and Affiliations

  1. Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA

    Jeannette S. Messer

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Gao, Y., Hall, C., MacLeod, J., Greer, P.A. (2019). Genetic Models of Calpain Deficiency and Ectopic Expression. In: Messer, J. (eds) Calpain. Methods in Molecular Biology, vol 1915. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-8988-1_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8988-1_20

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-8987-4

  • Online ISBN: 978-1-4939-8988-1

  • eBook Packages: Springer Protocols

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