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Pancreatic Cancer

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Gloria H. Su

    1. Department of Pathology and Cell Biology, Columbia University Medical Center, New York, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1882)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Subtyping of IPMN

    • Toru Furukawa
    Pages 1-8

  3. Clinical Management and Surgical Decision-Making of IPMN of the Pancreas

    • Masao Tanaka
    Pages 9-22

  4. Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous Neoplasm

    • Yongkang Kim, Taesung Park
    Pages 23-32

  5. Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma

    • Jungsun Kim, Kenneth S. Zaret
    Pages 33-53

  6. Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar-to-Ductal Metaplasia

    • Jun Liu, Pei Wang
    Pages 55-62

  7. Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial Neoplasia

    • James J. Lee, Seung K. Kim
    Pages 63-71

  8. Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform

    • Mackenzie Lee Goodwin, Sumi Kanthraj Urs, Diane M. Simeone
    Pages 73-80

  9. 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering

    • Claudio Ricci, Serena Danti
    Pages 81-95

  10. Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens

    • Lindsey A. Baker, Hervé Tiriac, David A. Tuveson
    Pages 97-115

  11. Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma

    • Lindsey A. Baker, David A. Tuveson
    Pages 117-133

  12. An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding

    • Takashi Okumura, Kenoki Ohuchida, Masafumi Nakamura
    Pages 135-141

  13. Osteoclast Differentiation Assay

    • Jingxuan Yang, Xiaohong Bi, Min Li
    Pages 143-148

  14. Primary Cultures for Pancreatic Stellate Cells (PSCs)

    • Lei Tian, Zipeng Lu, Yi Miao
    Pages 149-155

  15. Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells

    • Dong Qian, Lei Tian, Zipeng Lu, Yi Miao
    Pages 157-160

  16. Quantitative Method to Track Proteolytic Invasion in 3D Collagen

    • Kazumi Ebine, Christina R. Chow, Hidayatullah G. Munshi
    Pages 161-169

  17. Detection and Quantification of Macropinosomes in Pancreatic Tumors

    • Szu-Wei Lee, Basheer Alas, Cosimo Commisso
    Pages 171-181

  18. Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo

    • Edward Sim, Elena Irollo, Elda Grabocka
    Pages 183-195

  19. Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells

    • Chiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi, Ciro Isidoro
    Pages 197-206

  20. Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells

    • Yongde Luo, Dan Wang, James L. Abbruzzese, Weiqin Lu
    Pages 207-219
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Keywords

About this book

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have proven to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways of improving patient care for pancreatic cancer.

Editors and Affiliations

  • Department of Pathology and Cell Biology, Columbia University Medical Center, New York, USA

    Gloria H. Su

About the editor

   

Bibliographic Information

  • Book Title: Pancreatic Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Gloria H. Su

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8879-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8878-5Published: 31 October 2018

  • Softcover ISBN: 978-1-4939-9406-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8879-2Published: 30 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 337

  • Number of Illustrations: 11 b/w illustrations, 40 illustrations in colour

  • Topics: Cancer Research

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