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Xenopus pp 55–65Cite as

Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1865))

Abstract

Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward transcription activator-like effector nucleases (TALENs), adapted from plant pathogenic Xanthomonas species. Although functional genomics are more recently dominated by the even faster and more convenient CRISPR/Cas9 technology, the use of TALENs may still be preferred in a number of cases. We have successfully implemented this technology in Xenopus and in this chapter we describe our working protocol for targeted genome editing in X. tropicalis using TALENs.

The published version of the book has the family name of author that was erroneously captured as given name in the XML. This has now been updated.

The updated online version of this book can be found at https://doi.org/10.1007/978-1-4939-8784-9

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Acknowledgments

Research in the authors’ laboratory is supported by the Research Foundation–Flanders (FWO-Vlaanderen) (grants G0A1515N and G029413N), by the Belgian Science Policy (Interuniversity Attraction Poles—IAP7/07) and by the Concerted Research Actions from Ghent University (BOF15/GOA/011). Further support was obtained by the Hercules Foundation, Flanders (grant AUGE/11/14) and the Desmoid Tumor Research Foundation.

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Authors and Affiliations

  1. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium

    Tom Van Nieuwenhuysen & Kris Vleminckx

  2. Center for Medical Genetics, Ghent University, Ghent, Belgium

    Kris Vleminckx

  3. Cancer Research Institute Ghent, Ghent, Belgium

    Kris Vleminckx

Authors
  1. Tom Van Nieuwenhuysen
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  2. Kris Vleminckx
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Correspondence to Kris Vleminckx .

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Editors and Affiliations

  1. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium

    Kris Vleminckx

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Van Nieuwenhuysen, T., Vleminckx, K. (2018). Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8784-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8783-2

  • Online ISBN: 978-1-4939-8784-9

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