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Somatic Stem Cells pp 123–137Cite as

Markers and Methods to Study Adult Midgut Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1842))

Abstract

Stem cells have emerged as a promising cell source to heal, replace or regenerate tissue and organs damaged by aging, injury or diseases. The intestinal epithelium is the most rapidly renewing tissue in our body, which is maintained by intestinal stem cells (ISCs), located at the bottom of the crypts. ISCs continuously replace lost or injured intestinal epithelial cells in organisms ranging from Drosophila to humans. The adult Drosophila midgut provides an excellent in vivo model system to study ISC behavior during stress, regeneration, aging and infection. There are several signaling pathways/genes have been identified to regulate ISCs self-renewal and differentiation during normal and pathological conditions. A significant number of genetic tools and markers have been developed in the last one decade to study Drosophila ISCs behavior. Here, we describe some of the markers and methods used to study ISCs behavior in adult midgut of Drosophila.

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Author information

Authors and Affiliations

  1. Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, MD, USA

    Nathan Pinto, Beyoncé Carrington, Catharine Dietrich, Cristopher Aguilar, Tiffany Chen, Poonam Aggarwal & Shree Ram Singh

  2. National Cancer Institute, Frederick, MD, USA

    Rachit Sinha

  3. Department of Biology, University of Dayton, Dayton, Ohio, USA

    Madhuri Kango-Singh

Authors
  1. Nathan Pinto
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  2. Beyoncé Carrington
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  3. Catharine Dietrich
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  4. Rachit Sinha
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  5. Cristopher Aguilar
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  6. Tiffany Chen
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  7. Poonam Aggarwal
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  8. Madhuri Kango-Singh
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  9. Shree Ram Singh
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Correspondence to Shree Ram Singh .

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Editors and Affiliations

  1. Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, NJ, USA

    Pranela Rameshwar

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Pinto, N. et al. (2018). Markers and Methods to Study Adult Midgut Stem Cells. In: Singh, S., Rameshwar, P. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 1842. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8697-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8697-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8696-5

  • Online ISBN: 978-1-4939-8697-2

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