Skip to main content
SpringerLink
Log in

Microbial Proteomics

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Dörte Becher

    1. Department of Microbial Proteomics, Institute for Microbiology, University Greifswald, Greifswald, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of microbial proteomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1841)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Global Sample Preparation and Protein Enrichment

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Filter-Aided Sample Preparation for Proteome Analysis

      • Jacek R. WiÅ›niewski
      Pages 3-10

    3. Protein Enrichment from Highly Dilute Samples with StrataClean

      • Florian Bonn, Andreas Otto
      Pages 11-18

  3. Subcellular Fractionation

    1. Front Matter

      Pages 19-19
      Download chapter PDF

    2. Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins

      • Susanne Sievers
      Pages 21-33

    3. Enrichment of Cell Surface-Associated Proteins in Gram-Positive Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis

      • Florian Bonn, Sandra Maaß, Jan Maarten van Dijl
      Pages 35-43

    4. Preparation of Bacterial Magnetosomes for Proteome Analysis

      • Oliver Raschdorf, Dirk Schüler, René Uebe
      Pages 45-57

    5. Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics

      • Christian Manske, Ivo Finsel, Christine Hoffmann, Hubert Hilbi
      Pages 59-76

  4. Protein Quantification

    1. Front Matter

      Pages 77-77
      Download chapter PDF

    2. Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS

      • Martin Moche, Dirk Albrecht, Reiner Westermeier, Knut Büttner
      Pages 79-93

    3. Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms

      • Kathleen Trautwein, Ralf Rabus
      Pages 95-112

    4. Proteomic Signatures in Staphylococcus aureus

      • Susanne Engelmann, Stephan Fuchs
      Pages 113-130

    5. How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis

      • Alexander Reder, Stephan Michalik, Ulf Gerth
      Pages 131-140

    6. Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE

      • Sandra Maaß
      Pages 141-162

    7. Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses

      • Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, Frank Schmidt
      Pages 163-174

    8. Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)

      • Andreas Otto
      Pages 175-188

    9. Next-Generation Trapping of Protease Substrates by Label-Free Proteomics

      • Claudia Lindemann, Nikolas Thomanek, Katja Kuhlmann, Helmut E. Meyer, Katrin Marcus, Franz Narberhaus
      Pages 189-206

    10. In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings

      • Henrike Pförtner, Maren Depke, Kristin Surmann, Frank Schmidt, Uwe Völker
      Pages 207-228

  5. Analysis of Post Translational Protein Modifications

    1. Front Matter

      Pages 229-229
      Download chapter PDF
Back to top

Keywords

About this book

This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond.


Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Microbial Proteomics, Institute for Microbiology, University Greifswald, Greifswald, Germany

    Dörte Becher

Bibliographic Information

  • Book Title: Microbial Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Dörte Becher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8695-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8693-4Published: 27 September 2018

  • Softcover ISBN: 978-1-4939-9368-0Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8695-8Published: 26 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 344

  • Number of Illustrations: 24 b/w illustrations, 26 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation