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Flow Cytometry Protocols pp 301–319Cite as

Immunophenotypic Identification of Early Myeloerythroid Development

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1678))

Abstract

Myeloerythroid-restricted precursor cells, derived from multipotent hematopoietic stem cells, give rise to mature cells of the granulocyte, monocyte, erythroid, and/or thrombocytic lineages. High-resolution profiling of the developmental stages, from hematopoietic stem cells to mature progeny, is important to be able to study and understand the underlying mechanisms that guide various cell fate decisions. Also, this approach opens for greater insights into pathogenic events such as leukemia, diseases that are most often characterized by halted differentiation at defined immature precursor levels. In this chapter, we provide protocols and discuss approaches concerning the analysis and purification of immature myeloerythroid lineages by multiparameter flow cytometry. A wealth of literature has demonstrated the feasibility of similar approaches also for the human system. However, in this chapter, we focus on the identification of bone marrow cells derived from C57BL/6 mice, in which flow cytometry-based immunophenotypic applications have been most widely developed. This should allow also for its application in genetically modified models on this background. For maximal reproducibility, all protocols described have been established using reagents from commercial vendors to be analyzed on a flow cytometer with factory standard configuration.

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Acknowledgements

This work was supported by grants from the Swedish Cancer Foundation, the Swedish Medical Research Council, the Swedish Pediatric Childhood Cancer Foundation, and ERC consolidator grant 615068.

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Authors and Affiliations

  1. Division of Molecular Hematology, Institution for Laboratory Medicine, Lund University, Klinikgatan 26, 221 84, Lund, Sweden

    Cornelis J. H. Pronk & David Bryder

  2. Department of Pediatric Oncology/Hematology, Skane University Hospital, 221 85, Lund, Sweden

    Cornelis J. H. Pronk

Authors
  1. Cornelis J. H. Pronk
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  2. David Bryder
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Corresponding author

Correspondence to Cornelis J. H. Pronk .

Editor information

Editors and Affiliations

  1. Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

    Teresa S. Hawley

  2. Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, District of Columbia, USA

    Robert G. Hawley

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Pronk, C.J.H., Bryder, D. (2018). Immunophenotypic Identification of Early Myeloerythroid Development. In: Hawley, T., Hawley, R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology, vol 1678. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7346-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7346-0_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7344-6

  • Online ISBN: 978-1-4939-7346-0

  • eBook Packages: Springer Protocols

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