Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1657)
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Table of contents (34 protocols)
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Front Matter
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Detection and Quantitation of c-di-GMP
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Front Matter
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Visualizing c-di-GMP Levels Using Biosensors
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Front Matter
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Indirect Detection of c-di-GMP Levels
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Front Matter
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Modulation of c-di-GMP Levels and Bacterial Responses
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Front Matter
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About this book
This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling. The chapters, divided into eight major parts, guide readers through methods on synthesis, detection, quantitation, modulation of the levels of c-di-GMP present in cells, procedures to detect and evaluate the interaction of c-di-GMP, and up and coming approaches focusing on the inhibition of c-di-GMP signaling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Authoritative and cutting-edge, c-di-GMP Signaling: Methods and Protocols aims to inspire researchers to try new approaches.
Editors and Affiliations
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Department of Biological Sciences, Binghamton University Department of Biological Sciences, Binghamton, USA
Karin Sauer
Bibliographic Information
Book Title: c-di-GMP Signaling
Book Subtitle: Methods and Protocols
Editors: Karin Sauer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7240-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7239-5Published: 10 September 2017
Softcover ISBN: 978-1-4939-8427-5Published: 11 August 2018
eBook ISBN: 978-1-4939-7240-1Published: 09 September 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 486
Number of Illustrations: 32 b/w illustrations, 64 illustrations in colour
Topics: Cell Biology