Abstract
Plant ARGONAUTE (AGO) proteins regulate a wide range of cellular and developmental functions. Recent findings highlight their role during homologous recombination, a basic mechanism to repair double-strand DNA lesions (in somatic cells) and programmed DNA breaks (in meiocytes). This chapter contains an exhaustive description of procedures applied to analyze meiotic chromosome behavior (cytogenetic techniques) and DNA repair capacity (genotoxicity assays) in AGO-deficient Arabidopsis thaliana mutants.
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Acknowledgments
MMG and MP acknowledge the support of the European Union by the advanced grant Marie Curie Initial Training Network (ITN) “COMREC” (Grant agreement number: 606956). In addition, the work in MP laboratory is supported by a grant from the Ministerio de Economía y Competitividad of Spain (AGL2015–67349–P). The authors thank Juan Luis Santos and Eugenio Sánchez–Morán for helpful discussions about the importance of accurate chiasma quantification in the analysis of meiotic mutants, and Alexander Knoll and Holger Puchta for sharing protocols related to sensitivity assays using genotoxic agents.
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Martinez-Garcia, M., Pradillo, M. (2017). Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair. In: Carbonell, A. (eds) Plant Argonaute Proteins. Methods in Molecular Biology, vol 1640. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7165-7_10
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DOI: https://doi.org/10.1007/978-1-4939-7165-7_10
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