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  • © 2017

Mitochondria

Practical Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1567)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues

    • Jennifer Wettmarshausen, Fabiana Perocchi
    Pages 15-32
  3. Isolation of Mitochondria from Saccharomyces cerevisiae

    • Toshiaki Izawa, Ann-Katrin Unger
    Pages 33-42
  4. Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue

    • Bernadette Schreiner, Maria Ankarcrona
    Pages 53-68
  5. Chemical Crosslinking in Intact Mitochondria

    • Rupa Banerjee, Umut Günsel, Dejana Mokranjac
    Pages 139-154
  6. Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer

    • Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, Holger Prokisch
    Pages 217-230
  7. A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake

    • María Teresa Alonso, Paloma Navas-Navarro, Javier García-Sancho
    Pages 245-253
  8. Analysis of Yeast Mitochondria by Electron Microscopy

    • Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, Benedikt Westermann
    Pages 293-314
  9. Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography

    • Vicki A. M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, Martin van der Laan
    Pages 315-336

About this book

This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field.


Editors and Affiliations

  • Biomedical Center-Physiological Chemistry, LMU Munich, Martinsried, Germany

    Dejana Mokranjac

  • Gene Center, LMU Munich and Institute for Diabetes and Obesity, Helmholtz Zentrum München, Munich, Germany

    Fabiana Perocchi

Bibliographic Information

  • Book Title: Mitochondria

  • Book Subtitle: Practical Protocols

  • Editors: Dejana Mokranjac, Fabiana Perocchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6824-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6822-0Published: 11 March 2017

  • Softcover ISBN: 978-1-4939-8309-4Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6824-4Published: 07 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 409

  • Number of Illustrations: 31 b/w illustrations, 46 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access