Abstract
Lignin is a polyphenolic polymer specifically accumulating in the cell walls of xylem cells in higher vascular plants. Far from being homogeneous, the lignification of xylem cell walls varies in deposition site, quantity, composition and macromolecular conformation depending on the cell wall compartment, cell type, cell developmental stage and plant species. Here, we describe how confocal microspectroscopy methods using lignin autofluorescence can be used to evaluate the relative lignin amounts, its spatial distribution and composition at the cellular and sub-cellular levels in both isolated cells and histological cross-sections of plant tissues.
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References
Barros J, Serk H, Granlund I, Pesquet E (2015) The cell biology of lignification in higher plants. Ann Bot 115:1053–1074
Pomar F, Merino F, Barceló AR (2002) O-4-Linked coniferyl and sinapyl aldehydes in lignifying cell walls are the main targets of the Wiesner phloroglucinol-HCl reaction. Protoplasma 220:17–28
Crocker EC (1921) An experimental study of the significance of “lignin” color reactions. Ind Eng Chem 13:625–627
Gorzsás A, Stenlund H, Persson P, Trygg J, Sundberg B (2011) Cell specific chemotyping and multivariate imaging by combined FT-IR microspectroscopy and OPLS analysis reveals the chemical landscape of secondary xylem. Plant J 66:903–914
Pesquet E, Zhang B, Gorzsás A, Puhakainen T, Serk H, Escamez S, Barbier O, Gerber L, Courtois-Moreau C, Alatalo E, Paulin L, Kangasjärvi J, Sundberg B, Goffner D, Tuominen H (2013) Non-cell autonomous post-mortem lignification of tracheary elements in Zinnia elegans. Plant Cell 25:1314–1328
Donaldson L (1992) Lignin distribution during latewood formation in Pinus radiata D. Don. IAWA Bull 13:381–387
Fromm J, Rockel B, Lautner S, Windeisen E, Wanner G (2003) Lignin distribution in wood cell walls determined by TEM and backscattered SEM techniques. J Struct Biol 143:77–84
Terashima N, Fukushima K, Tsuchiya S, Takabe K (1986) Heterogeneity in formation of lignin: VII. An autoradiographic study on the formation of guaiacyl and syringyl lignin in poplar. J Wood Chem Technol 6:495–504
Terashima N, Fukushima K (1988) Heterogeneity in formation of lignin: XI. An autoradiographic study of the heterogeneous formation and structure of pine lignin. Wood Sci Technol 22:259–270
Terashima N, Fukushima K, Sano Y, Takabe K (1988) Heterogeneity in formation of lignin: X. Visualization of lignification process in differentiating xylem of pine by microautoradiography. Holzforschung 42:347–350
Fukushima K, Terashima N (1991) Heterogeneity in formation of lignin: Part XV. Formation and structure of lignin in compression wood of Pinus thunbergii studied by microautoradiography. Wood Sci Technol 25:371–381
Terashima N, Yoshida M, Hafrén J, Fukushima K, Westermark U (2012) Proposed supramolecular structure of lignin in softwood tracheid compound middle lamella regions. Holzforschung 66:907–915
Monardes N (1565) Historia medicinal de las cosas que se traen de nuestras Indias Occidentales que sirven en medicina. Sevilla
Djikanović D, Kalauzi A, Radotić K, Jeremić M (2007) Deconvolution of lignin fluorescent spectra: a contribution to the comparative structural studies of lignins. Russ J Phys Chem 81:1425–1428
Donaldson L, Radotić K, Kalauzi A, Djikanović D, Jeremić M (2010) Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. J Struct Biol 169:106–115
Albinsson B, Li S, Lundquist K, Stomberg R (1999) The origin of lignin fluorescence. J Mol Struct 50:19–27
Donaldson LA, Singh AP, Yoshinaga A, Takabe K (1999) Lignin distribution in mild compression wood of Pinus radiata. Can J Bot 77:41–50
Donaldson LA, Radotic K (2013) Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood. J Microsc 251:178–187
Donaldson LA (2013) Softwood and hardwood lignin fluorescence spectra of wood cell walls in different mounting media. IAWA J 34:3–19
Pesquet E, Tuominen H (2011) Ethylene stimulates tracheary element differentiation in Zinnia cell cultures. New Phytol 190:138–149
Zeng Y, Zhao S, Wei H, Tucker MP, Himmel ME, Mosier NS, Meilan R, Ding SY (2015) In situ micro-spectroscopic investigation of lignin in poplar cell walls pretreated by maleic acid. Biotechnol Biofuels 8:126–138
Serk H, Gorzsás A, Tuominen H, Pesquet E (2015) Cooperative lignification of xylem tracheary elements. Plant Signal Behav 10:e1003753
Nakaba S, Yamagishi Y, Sano Y, Funada R (2012) Temporally and spatially controlled death of parenchyma cells is involved in heartwood formation in pith regions of branches of Robinia pseudoacacia var. inermis. J Wood Sci 58:69–76
Radotić K, Kalauzi A, Djikanović D, Jeremić M, Leblanc RM, Cerović ZG (2006) Component analysis of the fluorescence spectra of a lignin model compound. J Photochem Photobiol 83:1–10
Ménard D, Serk H, Decou R, Pesquet E (2016) Establishment and utilization of habituated cell suspension cultures for hormone-inducible xylogenesis. In: Xylem. Springer, New York
Acknowledgment
This work was supported by Vetenskapsrådet VR (grant 2010-4620 to E.P.), the BioImprove FORMAS (to E.P.), the Berzelii Centre for Forest Biotechnology (to D.M. and E.P.), by the Kempe Foundation (Gunnar Öquist Fellowship to E.P.), by the Carl Tryggers Foundation and by the Stiftelsen för Strategisk Forskning (ValueTree) to E.P. We also thank the Swedish research council Vetenskapsrådet and the Swedish Governmental Agency for Innovation Systems Vinnova (UPSC Berzelii Centre) and Bio4Energy (a strategic research environment appointed by the Swedish government).
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Decou, R., Serk, H., Ménard, D., Pesquet, E. (2017). Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy. In: de Lucas, M., Etchhells, J. (eds) Xylem. Methods in Molecular Biology, vol 1544. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6722-3_17
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DOI: https://doi.org/10.1007/978-1-4939-6722-3_17
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